Table of Contents

CFI-400945 25μM R08 exp469

Mechanism of Action

Inhibits PLK4, blocks centriole duplication, induces mitotic defects and cell death

Technical Notes

Compound References

  • PubChem Name: CFI-400945 free base
  • Synonyms: N/A
  • CAS #: 1338806-73-7
  • PubChem CID: 58486178
  • IUPAC: (2'S,3R)-2'-[3-[(E)-2-[4-[[(2S,6R)-2,6-dimethylmorpholin-4-yl]methyl]phenyl]ethenyl]-1H-indazol-6-yl]-5-methoxyspiro[1H-indole-3,1'-cyclopropane]-2-one
  • INCHI Name: InChI=1S/C33H34N4O3/c1-20-17-37(18-21(2)40-20)19-23-6-4-22(5-7-23)8-12-29-26-11-9-24(14-31(26)36-35-29)28-16-33(28)27-15-25(39-3)10-13-30(27)34-32(33)38/h4-15,20-21,28H,16-19H2,1-3H3,(H,34,38)(H,35,36)/b12-8+/t20-,21+,28-,33-/m0/s1
  • INCHI Key: DADASRPKWOGKCU-FVTQAUBDSA-N
  • Molecular Weight: 534.6
  • Canonical SMILES: CC1CN(CC(O1)C)CC2=CC=C(C=C2)C=CC3=NNC4=C3C=CC(=C4)C5CC56C7=C(C=CC(=C7)OC)NC6=O
  • Isomeric SMILES: C[C@@H]1CN(C[C@@H](O1)C)CC2=CC=C(C=C2)/C=C/C3=NNC4=C3C=CC(=C4)[C@@H]5C[C@]56C7=C(C=CC(=C7)OC)NC6=O
  • Molecular Formula: C33H34N4O3

Compound Supplier

  • Supplier Name: Med Chem Express
  • Catalog #: HY-12300
  • Lot #: 23771

Compound Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C33H34N4O3 535.27037; found 535.27075

Dose Response Curve

  • Platform ID: CFI400945
  • Min: -8.6037; Max: 76.6696





IC Concentration (µM)
IC10 19.9700
IC20 24.7200
IC30 28.4900
IC40 32.0000
IC50 35.6100
IC60 39.6200
IC70 44.5100
IC80 51.3000
IC90 63.5100


Screen Summary

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
29/0ScoresViewViewViewViewView