Ammonium tetrathiomolybdate 10μM R08 exp448

Ammonium tetrathiomolybdate 10μM R08 exp448

Mechanism of Action

Inhibits cuproenzymes superoxide dismutase 1 (SOD1) and cytochrome c oxidase (COX)

Class / Subclass 1: Environmental Stresses / Metal Chelator
Class / Subclass 2: Metabolism / Metabolic Enzyme Inhibitor

Technical Notes

Protein References

PubChem Name: Ammonium tetrathiomolybdate
Synonyms: N/A
CAS #: 15060-55-6
PubChem CID: 15251598
IUPAC: diazanium;bis(sulfanylidene)molybdenum;sulfanide
INCHI Name: InChI=1S/Mo.2H3N.2H2S.2S/h;2*1H3;2*1H2;;
INCHI Key: ZKKLPDLKUGTPME-UHFFFAOYSA-N
Molecular Weight: 262.3
Canonical SMILES: [NH4+].[NH4+].[SH-].[SH-].S=[Mo]=S
Isomeric SMILES: N/A
Molecular Formula: H10MoN2S4

Protein Supplier

Supplier Name: Sigma-Aldrich
Catalog #: 323446
Lot #: MKCG8991

Protein Characterization

XRPD: Expected (NH4)2MoS4; found (NH4)2MoS4

Dose Response Curve

Platform ID: AMTT
Min: -10.9997; Max: 86.4825

IC	Concentration (µM)
IC10	0.6222
IC20	1.7960
IC30	3.6350
IC40	6.4770
IC50	11.0100
IC60	18.7000
IC70	33.3300
IC80	67.4300
IC90	194.7000

Screen Summary

Round: 08
Dose: 10µM
Days of incubation: 8
Doublings: 0.4
Numbers of reads: 16291905

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/35	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
PSMA7	-3.19	0.20
EIF3B	-2.70	0.43
COPB1	-2.64	0.43
ATP6V0B	-2.64	0.46
ATP6AP1	-2.48	0.50
PDGFC	-2.41	0.24
SLC29A1	-2.40	0.24
PLK1	-2.39	0.50
DDX21	-2.38	0.46
PRDM4	-2.34	0.26
DHX38	-2.33	0.46
UACA	-2.30	0.26
POLA1	-2.29	0.47
DDX46	-2.28	0.52
CDC73	-2.27	0.43
CARF	-2.26	0.41
RPS11	-2.24	0.46
PLK4	-2.22	0.43
TINF2	-2.22	0.43
IAPP	-2.20	0.39
PRKAA1	-2.16	0.41
GOLGA6L1	-2.16	0.62
HMGCR	-2.16	0.50
EEFSEC	-2.12	0.41
CHMP2A	-2.12	0.47
GOLGA6L6	-2.11	0.46
NKX6-1	-2.11	0.46
POLR1D	-2.10	0.43
SEPHS2	-2.08	0.43
NUPL1	-2.08	0.43
TP53	2.60	0.02
COQ3	2.68	<0.01
ATP5A1	2.68	0.03
NDUFA2	2.69	0.01
MFSD9	2.79	<0.01
TKT	2.81	0.01
COQ5	2.82	<0.01
FASTKD1	2.90	<0.01
DOHH	2.93	<0.01
TAZ	3.05	0.04
PPP6C	3.13	<0.01
UQCRC1	3.14	<0.01
COQ6	3.14	<0.01
COQ2	3.14	<0.01
MED16	3.19	<0.01
ME2	3.27	0.00
COQ4	3.31	0.00
UQCRQ	3.43	0.00
AASDHPPT	3.45	0.00
COQ7	3.51	0.00
MBTPS2	3.53	0.00
PDSS2	3.54	0.00
BAK1	3.63	0.00
UQCC1	3.68	0.00
BCS1L	3.74	0.00
UQCRC2	3.77	0.00
PDSS1	3.80	0.00
UQCC2	4.40	0.00
ATOX1	6.64	0.00
ATP7A	8.49	0.00

Screen	Correlation	Plot
Atovaquone 15μM R08 exp451	0.136	View
Vinblastine 0.2μM R03 exp108	0.091	View
Bleomycin 5μM R08 exp459	0.085	View
Tanespimycin 14μM R08 exp527	0.083	View
Doxorubicin 0.02μM R08 exp478	0.081	View
Hinokiflavone 12μM R08 exp487	0.079	View
GSK461364A 0.1μM R03 exp126	0.078	View
CI-1040 9.5μM R08 exp472	0.077	View
SNS-032 15μM R08 exp443	0.076	View
Benzoate 20000μM R08 exp456	0.075	View
Artemisinin 50μM R08 exp450	0.073	View
Aphidicolin 0.4μM R08 exp440	0.071	View
BI-2536 0.02μM R03 exp96	0.071	View
Monensin 0.3μM R08 exp507	0.07	View
TIC10 10μM R08 exp532	0.07	View
ONC212 0.15μM R08 exp513	0.066	View
Mitomycin-C 0.06μM R08 exp503	0.063	View
Etoposide 0.3μM R05 exp199	0.063	View
THZ1 0.06μM R08 exp531	0.062	View
MS023 7μM R03 exp135	0.062	View
GSK-J4 1.5μM R08 exp441	0.06	View
UM131593 0.2μM R03 exp106	0.06	View
BI-6727 0.04μM R03 exp98	0.056	View
CR131-b 0.005μM R08 exp474	0.055	View
MLN-4924 2μM R00 exp22	0.054	View
PU-H71 1μM R08 exp515	0.052	View
Hippuristanol 0.12μM R08 exp489 no dilution day6	0.052	View
42°C R08 exp539	0.078	View
AICAR 240μM R05 exp211	0.065	View
5-Fluorouracil 20μM R00 exp2	0.059	View
Actinomycin-D 0.001μM R00 exp3	0.053	View

GO Term	Fold Change	Genes
ubiquinone biosynthesis complex	552.30	COQ7,COQ4,COQ6,COQ5,COQ3
extrinsic component of mitochondrial inner membrane	368.20	COQ7,COQ4,COQ5,COQ3
ubiquinone biosynthetic process	294.56	PDSS1,PDSS2,COQ7,COQ4,COQ2,COQ6,COQ5,COQ3
ubiquinone metabolic process	259.91	PDSS1,PDSS2,COQ7,COQ4,COQ2,COQ6,COQ5,COQ3
quinone biosynthetic process	257.74	PDSS1,PDSS2,COQ4,COQ2,COQ6,COQ5,COQ3
respiratory chain complex III	245.47	UQCRC2,BCS1L,UQCRQ,UQCRC1
ketone biosynthetic process	157.80	PDSS1,PDSS2,COQ7,COQ4,COQ2,COQ6,COQ5,COQ3
quinone metabolic process	119.42	PDSS1,PDSS2,COQ7,COQ4,COQ2,COQ6,COQ5,COQ3
extrinsic component of organelle membrane	102.28	COQ7,COQ4,COQ6,COQ5,COQ3
cellular ketone metabolic process	52.60	PDSS1,PDSS2,COQ7,COQ4,COQ2,COQ6,COQ5,COQ3
respiratory chain complex	44.78	UQCRC2,BCS1L,UQCRQ,UQCRC1,NDUFA2,NDUFB11
mitochondrial respirasome	44.18	UQCRC2,BCS1L,UQCRQ,UQCRC1,NDUFA2,NDUFB11
respirasome	40.41	UQCRC2,BCS1L,UQCRQ,UQCRC1,NDUFA2,NDUFB11
inner mitochondrial membrane protein complex	29.29	UQCRC2,BCS1L,UQCRQ,UQCRC1,NDUFA2,ATP5F1A,NDUFB11
mitochondrial inner membrane	18.09	UQCC2,UQCRC2,BCS1L,UQCC1,COQ7,UQCRQ,COQ4,COQ2,COQ6,UQCRC1,COQ5,NDUFA2,ATP5F1A,COQ3,NDUFB11
organelle inner membrane	16.24	UQCC2,UQCRC2,BCS1L,UQCC1,COQ7,UQCRQ,COQ4,COQ2,COQ6,UQCRC1,COQ5,NDUFA2,ATP5F1A,COQ3,NDUFB11
mitochondrial membrane	13.32	UQCC2,UQCRC2,BCS1L,UQCC1,BAK1,COQ7,UQCRQ,COQ4,COQ2,COQ6,UQCRC1,COQ5,NDUFA2,ATP5F1A,COQ3,VDAC2,NDUFB11
mitochondrial envelope	12.50	UQCC2,UQCRC2,BCS1L,UQCC1,BAK1,COQ7,UQCRQ,COQ4,COQ2,COQ6,UQCRC1,COQ5,NDUFA2,ATP5F1A,COQ3,VDAC2,NDUFB11
mitochondrial matrix	11.65	UQCC2,PDSS1,PDSS2,ME2,FASTKD1,COQ5,ATP5F1A,COQ3,TP53,VDAC2

Table of Contents

Ammonium tetrathiomolybdate 10μM R08 exp448

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results