Table of Contents

Q15 2.7μM R02 exp79

Q15 2.7μM R02 exp79

Mechanism of Action

Putative condensin inhibitor

Class / Subclass 1: Cell Cycle / Chromosome Dynamics Inhibitor

Technical Notes

Protein References

PubChem Name: N/A
Synonyms: N/A
CAS #: 1071780-64-7
PubChem CID: N/A
IUPAC: N/A
INCHI Name: N/A
INCHI Key: N/A
Molecular Weight: 584.0421
Canonical SMILES: N/A
Isomeric SMILES: N/A
Molecular Formula: C31H27ClFN7O2

Protein Supplier

Supplier Name: In house - University of Montreal - Chemistry Platform
Catalog #: N/A
Lot #: N/A

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C31H27ClFN7O2 584.19716; found 584.19427

Dose Response Curve

Platform ID: Q15
Min: -15.2028; Max: 99.7587

IC	Concentration (µM)
IC10	N/A
IC20	2.4826
IC30	2.6903
IC40	2.8735
IC50	3.0525
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 02
Dose: 2.7µM
Days of incubation: 8
Doublings: 0.6
Numbers of reads: 11718749

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
23/34	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
VKORC1L1	-4.08	<0.01
ACBD3	-3.95	<0.01
DDX23	-3.63	0.03
OSBPL11	-3.62	<0.01
LDLR	-3.53	<0.01
SPTLC2	-3.52	<0.01
CHKA	-3.40	<0.01
OSBPL8	-3.33	<0.01
ATP6V0A2	-3.31	<0.01
OSBPL9	-3.24	<0.01
UPF2	-3.04	0.23
HSPE1	-3.03	0.06
COMMD5	-2.76	<0.01
AP5S1	-2.69	0.01
COL4A3BP	-2.62	0.01
ATP6V1B2	-2.56	0.04
RPS14	-2.54	0.10
FLVCR1	-2.51	0.04
PCYT1A	-2.51	0.03
RAB1B	-2.51	0.03
SCAP	-2.47	0.04
DAGLB	-2.46	0.03
PSMA5	-2.45	0.22
CLVS1	-2.45	0.03
RPL18A	-2.45	0.03
WDR7	-2.43	0.06
COPB1	-2.42	0.23
SCYL3	-2.41	0.06
UNC50	-2.40	0.04
TRAPPC13	-2.39	0.04
RFK	2.43	0.02
PGLS	2.46	0.02
ZNF562	2.48	0.02
ATP5L	2.50	0.03
C12orf44	2.59	0.01
WSB1	2.59	0.14
LCLAT1	2.60	0.01
DNAJC3	2.62	<0.01
SLC20A1	2.69	0.01
ZCRB1	2.70	0.01
PI4K2B	2.79	<0.01
WIBG	2.97	0.02
INSIG1	2.98	<0.01
CDS2	3.00	0.02
ELF1	3.03	<0.01
SP1	3.05	<0.01
RPE	3.16	<0.01
ENY2	3.21	<0.01
NONO	3.28	0.00
PFKP	3.39	0.00
SLC5A3	3.52	0.00
TKT	3.56	0.00
UBE2G1	3.61	0.00
GPI	3.63	<0.01
ACACA	4.27	0.00
HK2	4.31	0.00
CHP1	5.29	0.00
PGP	5.60	0.00
AGPAT6	6.13	0.00
KCTD5	6.16	0.00

Screen	Correlation	Plot
Vemurafenib 6.6μM R02 exp89	0.101	View
Vemurafenib 6.6μM R08 exp434	0.089	View
UM0125461 0.74μM R02 exp84	0.069	View
WYE-354 6μM R02 exp90	0.054	View
Q15 1 to 2μM on day4 R04 exp164	0.164	View
Clomiphene 4.4μM R02 exp68	0.102	View
BH1 1μM R04 exp169	0.073	View
Bafilomycin-A1 0.009μM R08 exp454	0.072	View
CFI-400945 25μM R08 exp469	0.059	View
∆-9-Tetrahydrocannabinol 30μM R08 exp445	0.053	View
Bortezomib 0.05μM R00 exp7	0.051	View
FTY720 3μM R08 exp483	0.051	View

GO Term	Fold Change	Genes
pentose-phosphate shunt, non-oxidative branch	221.12	TKT,RPE
CDP-diacylglycerol biosynthetic process	138.20	GPAT4,CDS2,LCLAT1
CDP-diacylglycerol metabolic process	138.20	GPAT4,CDS2,LCLAT1
pentose-phosphate shunt	138.20	TKT,RPE,PGLS
glucose 6-phosphate metabolic process	138.20	HK2,GPI,TKT,RPE,PGLS
NADPH regeneration	110.56	TKT,RPE,PGLS
generation of precursor metabolites and energy	11.76	HK2,GPI,TKT,PFKP,RPE,ATP5MG,PGLS,NDUFB11
glycerolipid metabolic process	9.76	GPAT4,PGP,CDS2,INSIG1,PI4K2B,LCLAT1
purine nucleotide metabolic process	9.70	GPAT4,ACACA,TKT,RPE,ATP5MG,PGLS,NDUFB11
nucleotide metabolic process	9.66	GPAT4,ACACA,TKT,RPE,ATP5MG,PGLS,RFK,NDUFB11
organophosphate metabolic process	9.60	GPAT4,PGP,HK2,ACACA,GPI,TKT,PFKP,RPE,CDS2,PI4K2B,LCLAT1,ATP5MG,PGLS,RFK,NDUFB11
organophosphate biosynthetic process	9.59	GPAT4,ACACA,TKT,CDS2,PI4K2B,LCLAT1,ATP5MG,RFK,NDUFB11
nucleoside phosphate metabolic process	9.47	GPAT4,ACACA,TKT,RPE,ATP5MG,PGLS,RFK,NDUFB11
purine-containing compound metabolic process	9.06	GPAT4,ACACA,TKT,RPE,ATP5MG,PGLS,NDUFB11
nucleobase-containing small molecule metabolic process	8.25	GPAT4,ACACA,TKT,RPE,ATP5MG,PGLS,RFK,NDUFB11
carbohydrate derivative metabolic process	6.64	GPAT4,HK2,ACACA,GPI,TKT,PFKP,RPE,ATP5MG,PGLS,RFK,NDUFB11