MOCS3

Gene Information

Official Symbol: MOCS3
Official Name: molybdenum cofactor synthesis 3
Aliases and Previous Symbols: N/A
Entrez ID: 27304
UniProt: O95396
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. {ECO:0000255|HAMAP-Rule:MF_03049, ECO:0000269|PubMed:15073332, ECO:0000269|PubMed:19017811}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Cadmium 60μM R08 exp462	-2.53
ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319	-2.52
BML-284 0.09μM R08 exp460	-2.45
CCCP 0.1μM R00 exp10	-2.4
THZ531 0.11 to 0.125μM on day4 R07 exp410	-2.3
THZ531 0.11 to 0.175μM on day4 R07 exp413	-2.26
SB202190 10μM R03 exp148	-2.24
A-395N 10μM R05 exp219	-2.22
Erlotinib 10μM R05 exp216	-2.2
Dimethyloxaloylglycine 100μM R05 exp229	-2.14
Geldanamycin 0.015 to 0.025μM on day4 R07 exp316	-2.13
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	-2.11
VER-155008 3.9μM R06 exp301	-2.08
PNU96415E 10μM R03 exp145	-2.07
Hydroxychloroquine 30μM R08 exp490	-1.95
UM0011500 5μM R05 exp245	-1.95
S-trityl-L-cysteine 0.5μM R05 exp243	-1.89
Vincristine 0.0005μM R04 exp190	-1.86
THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412	-1.83
35°C R06 exp302	-1.8
L-BMAA 500 to 750μM on day4 R04 exp185	-1.8
Alpha-Amanitin 0.5μM R00 exp5	-1.79
THZ531 0.11μM R07 exp409	-1.77
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	-1.72
GSK343 3μM R03 exp124	-1.71
Dexamethasone 0.006μM R07 exp351	-1.7
Adefovir 20μM R07 exp332	-1.7
Phenformin 20μM R05 exp212	1.73
Nocodazole 0.2μM R02 exp64	1.85
AICAR 240μM R03 exp116	1.92
Phenformin 20μM R03 exp143	1.92
iCRT14 30μM R08 exp492	1.93
LY2090314 0.003μM R08 exp500 no dilution day6	1.94
CR131-b 0.005μM R08 exp474	1.95
Thapsigargin 0.005μM R07 exp407	1.98
UM0129480 7μM R02 exp85	2.01
Mubritinib 0.2μM R01 exp48	2.02
Tanespimycin 14μM R08 exp527	2.12
Amiloride 100μM R08 exp447	2.21
SNS-032 15μM R08 exp443	2.23
QNZ 0.01μM R08 exp439	2.27
Vinblastine 0.2μM R03 exp108	2.32
6-Thio-2-deoxyguanosine 2μM R06 exp259	2.41
R-DABN 8μM R03 exp111	2.45
Hippuristanol 0.12μM R08 exp489 no dilution day6	2.68
Rotenone 0.07μM R08 exp431	2.78
Milciclib 2μM R08 exp502	2.83
Artemisinin 50μM R08 exp450	3.06
MS023 7μM R03 exp135	3.09
2-Deoxy-D-glucose 800μM R05 exp214	3.46
MLN-4924 0.2μM R00 exp21	3.58
MS023 2μM R03 exp134	3.63
Hippuristanol 0.12μM R08 exp488	3.73

Gene	Correlation
URM1	0.777
CTU1	0.76
ELP6	0.706
ELP3	0.696
DPH3	0.641
KTI12	0.629
CTU2	0.628
ELP2	0.628
ELP4	0.573
ELP5	0.543
IKBKAP	0.528
THG1L	0.495
KDSR	0.493
NR2C2AP	0.492
DPH1	0.483
SSB	0.477
ASUN	0.471
MAPKAP1	0.47
SBDS	0.469
ATIC	0.469
DOHH	0.465
GEMIN6	0.463
DPH5	0.457
N6AMT1	0.455
DHRSX	0.45
MSTO1	0.439
SLC7A1	0.439
ALKBH8	0.438
C15orf41	0.437
VEZT	0.435
DR1	0.432
RPP21	0.43
ATXN10	0.429
TRMT61A	0.427
INTS10	0.426
ASB3	0.424
DOLK	0.421
TSEN54	0.421
MLST8	0.419
PGM3	0.413
BCS1L	0.412
C7orf26	0.412
FAM98B	0.41
SEC62	0.409
RPTOR	0.409
TEN1	0.407
TKT	0.407
DNLZ	0.405
CD81	0.401
PPCS	0.401
TRMT10A	0.401
PIK3CD	0.4

Global Fraction of Cell Lines Where Essential: 286/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	12/28
blood	11/28
bone	11/26
breast	7/33
central nervous system	29/56
cervix	0/4
colorectal	6/17
esophagus	6/13
fibroblast	0/1
gastric	2/16
kidney	11/21
liver	8/20
lung	29/75
lymphocyte	5/16
ovary	7/26
pancreas	8/24
peripheral nervous system	4/16
plasma cell	3/15
prostate	0/1
skin	15/24
soft tissue	4/9
thyroid	0/2
upper aerodigestive	7/22
urinary tract	13/29
uterus	3/5

Essentiality in NALM6

Essentiality Rank: 2126
Expression level (log2 read counts): 4.14

Expression Distribution

MoeZ MoeB
Rhodanese
ThiF

molybdopterin-synthase adenylyltransferase activity
enzyme active site formation via cysteine modification to L-cysteine persulfide
peptidyl-cysteine modification to L-cysteine persulfide
molybdopterin-synthase sulfurtransferase activity
URM1 activating enzyme activity
enzyme active site formation
sulfurtransferase activity
thiosulfate sulfurtransferase activity
protein urmylation
tRNA wobble position uridine thiolation
tRNA thio-modification
Mo-molybdopterin cofactor biosynthetic process
Mo-molybdopterin cofactor metabolic process
molybdopterin cofactor biosynthetic process
molybdopterin cofactor metabolic process
prosthetic group metabolic process
nucleotidyltransferase activity
tRNA wobble uridine modification
tRNA wobble base modification
peptidyl-cysteine modification
tRNA modification
tRNA processing
coenzyme biosynthetic process
RNA modification
tRNA metabolic process
cofactor biosynthetic process
coenzyme metabolic process
ncRNA processing
cofactor metabolic process
ncRNA metabolic process
organophosphate biosynthetic process
protein modification by small protein conjugation
RNA processing
peptidyl-amino acid modification
organophosphate metabolic process
protein modification by small protein conjugation or removal
heterocycle biosynthetic process
organic cyclic compound biosynthetic process
organonitrogen compound biosynthetic process
ATP binding