DPH5

Gene Information

Official Symbol: DPH5
Official Name: diphthamide biosynthesis 5
Aliases and Previous Symbols: N/A
Entrez ID: 51611
UniProt: Q9H2P9
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis. {ECO:0000250|UniProtKB:P32469, ECO:0000269|PubMed:23486472}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
GSK461364A 0.005μM R03 exp125	-2.81
Dexamethasone 0.006μM R07 exp351	-2.48
Vincristine 0.0005μM R04 exp190	-2.3
Colchicine 0.009μM R05 exp215	-2.27
Demecolcine 0.03μM R05 exp228	-2.27
Zebularine 20μM R07 exp423	-2.05
Combretastatin A4 0.002 to 0.003μM day4 R04 exp179	-2
Apcin 50 to 100μM on day4 R04 exp176	-1.96
Ethambutol 25μM R08 exp481	-1.85
BH1 1μM R04 exp169	-1.75
Mubritinib 0.2μM R02 exp65	1.72
Pifithrin-alpha 20μM R07 exp390	1.72
Oligomycin-A 2μM R00 exp25	1.75
Sulforaphane 9μM R08 exp525	1.78
Calcium Ionophore 0.4μM R07 exp342	1.79
IU1-C 25μM R04 exp183	1.79
R-DABN 8μM R03 exp111	1.79
Staurosporine 0.02μM R08 exp524	1.81
AICAR 240μM R05 exp211	1.82
Chloramphenicol 20μM R00 exp13	1.84
UM0131023 0.05μM R05 exp248	1.87
Docetaxel 0.002μM R01 exp45	1.9
QNZ 0.01μM R08 exp439	1.91
Dimethyl-Sulfoxide 1pc R03 exp120	1.93
2-Deoxy-D-glucose 800μM R05 exp214	1.95
FK-506 30μM R07 exp359	1.96
Trientine 500μM R08 exp534	1.96
Trimetrexate 0.03μM R08 exp535	1.97
Temozolomide 200μM R04 exp189	2
iCRT14 30μM R08 exp492	2.02
Deguelin 0.15μM R05 exp209	2.04
GSK-J4 1.5μM R08 exp441	2.05
I-BRD9 4μM R07 exp365	2.28
Citral 75μM R06 exp275	2.31
Olaparib 4μM R08 exp512	2.35
Taxol 0.004μM R03 exp103	2.41
AICAR 240μM R03 exp116	2.54
UM0125461 0.74μM R02 exp84	2.57
Hippuristanol 0.12μM R08 exp489 no dilution day6	2.78
Hippuristanol 0.12μM R08 exp488	2.8
CB-5083 0.4μM R08 exp468	2.86
Bortezomib 0.005μM R00 exp6	3.04
MS023 7μM R03 exp135	3.25
Citral 50μM R06 exp274	3.3
GSK-J4 1-1.25μM to day4 R07 exp363	3.4
MS023 2μM R03 exp134	3.4
UM0129480 7μM R02 exp85	4.01

Gene	Correlation
DPH1	0.759
DPH6	0.694
DNAJC24	0.692
DPH2	0.685
DPH3	0.535
MAPKAP1	0.474
URM1	0.469
ELP2	0.458
MOCS3	0.457
PIK3CD	0.456
CTU1	0.437
CD81	0.437
KTI12	0.437
CD19	0.428
NR2C2AP	0.427
ELP6	0.424
CXorf56	0.417
YTHDF2	0.416
ELP3	0.415
ZZZ3	0.411
PRIM1	0.401
SLC7A1	0.4
KDSR	0.4

Global Fraction of Cell Lines Where Essential: 5/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	1/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	2/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 9812
Expression level (log2 read counts): 5.29

Expression Distribution

diphthine synthase activity
peptidyl-diphthamide metabolic process
peptidyl-diphthamide biosynthetic process from peptidyl-histidine
peptidyl-histidine modification
regulation of cytoplasmic translational elongation
regulation of translational elongation
regulation of cytoplasmic translation
methylation
regulation of translation
regulation of cellular amide metabolic process
cellular component
posttranscriptional regulation of gene expression
peptidyl-amino acid modification