UM0129480 7μM R02 exp85

UM0129480 7μM R02 exp85

Mechanism of Action

Unknown

Class / Subclass 1: Uncharacterized Mechanism / Chemical Screen Hit

Technical Notes

Protein References

PubChem Name: N/A
Synonyms: N/A
CAS #: N/A
PubChem CID: N/A
IUPAC: N/A
INCHI Name: N/A
INCHI Key: N/A
Molecular Weight: 565.43
Canonical SMILES: N/A
Isomeric SMILES: N/A
Molecular Formula: C27H21N2O2F3SCl2

Protein Supplier

Supplier Name: In house - University of Montreal - Chemistry Platform
Catalog #: N/A
Lot #: IRIC-004 / 2119-072-1 Rico Lavo / Batch #01

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C27H21Cl2F3N2O2S 565.07257; found 565.07328

Dose Response Curve

Platform ID: UM129480
Min: -12.7498; Max: 77.5794

IC	Concentration (µM)
IC10	N/A
IC20	4.6558
IC30	5.4831
IC40	6.2699
IC50	7.0908
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 02
Dose: 7µM
Days of incubation: 8
Doublings: 1.5
Numbers of reads: 13412153

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
15/42	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
SLC29A1	-4.40	<0.01
TCF4	-3.78	<0.01
PRKAG1	-3.60	<0.01
PRKAA1	-3.09	<0.01
OTUB1	-2.99	<0.01
WDR26	-2.99	0.02
SMG7	-2.85	0.05
TRIM44	-2.82	0.02
ATF4	-2.81	0.02
TRAF2	-2.78	0.02
PRAMEF25	-2.68	0.43
PRAMEF26	-2.67	0.44
BSG	-2.61	0.04
IPO11	-2.60	0.02
YPEL5	-2.54	0.03
RPL30	-2.53	0.13
BCOR	-2.50	0.03
SMG9	-2.46	0.05
GLE1	-2.46	0.40
TBK1	-2.45	0.05
GP2	-2.43	0.04
TSC2	-2.40	0.05
MBTD1	-2.40	0.06
ATP13A1	-2.38	0.05
RING1	-2.37	0.05
SLC2A13	-2.35	0.05
MDM2	-2.32	0.14
PRKACG	-2.31	0.05
PCYT1A	-2.29	0.06
TCF12	-2.29	0.10
SEPSECS	2.58	0.04
ELP6	2.58	<0.01
THG1L	2.60	<0.01
VDAC2	2.66	<0.01
COA3	2.67	<0.01
PMAIP1	2.67	<0.01
GLRX5	2.67	0.06
ZCRB1	2.67	0.01
DDX39A	2.71	<0.01
PUS3	2.75	<0.01
FBXO11	2.88	<0.01
TXNIP	2.89	<0.01
RFK	2.93	<0.01
KCTD5	3.10	<0.01
OMA1	3.25	0.00
ENY2	3.31	<0.01
CMAS	3.33	0.00
IBA57	3.35	0.00
NFU1	3.40	<0.01
DPH2	3.47	0.00
STARD7	3.60	0.00
PTAR1	3.73	0.00
UBQLN1	3.87	0.00
BAK1	3.93	0.00
DPH5	4.01	0.00
DPH1	4.13	0.00
PPP6C	4.46	0.00
DPH3	4.57	0.00
RBM26	4.69	0.00
MARCH5	4.97	0.00

Screen	Correlation	Plot
iCRT14 30μM R08 exp492	0.09	View
Wortmannin 5μM R00 exp38	0.07	View
6-Thio-2-deoxyguanosine 2μM R06 exp259	0.097	View
Artemisinin 50μM R08 exp450	0.094	View
Dorsomorphin 5μM R07 exp357	0.079	View
AICAR 240μM R03 exp116	0.078	View
Bleomycin 5μM R08 exp459	0.075	View
Atovaquone 15μM R08 exp451	0.075	View
RO-3307 4.7μM R02 exp80	0.071	View
MS023 2μM R03 exp134	0.071	View
Actinomycin-D 0.001μM R00 exp3	0.071	View
Benzoate 20000μM R08 exp456	0.07	View
Hippuristanol 0.12μM R08 exp488	0.069	View
MS023 7μM R03 exp135	0.069	View
GSK626616 14μM R08 exp485	0.069	View
Etoposide 0.3μM R05 exp199	0.068	View
CI-1040 9.5μM R08 exp472	0.067	View
SNS-032 15μM R08 exp443	0.067	View
Milciclib 2μM R08 exp502	0.066	View
BVD-523 15μM R02 exp67	0.066	View
Aphidicolin 0.4μM R08 exp440	0.064	View
Mitomycin-C 0.06μM R08 exp503	0.064	View
2-Deoxy-D-glucose 800μM R05 exp214	0.063	View
Olaparib 4μM R08 exp512	0.063	View
PU-H71 1μM R08 exp515	0.061	View
GSK-J4 1-1.25μM to day4 R07 exp363	0.061	View
Staurosporine 0.02μM R08 exp524	0.061	View
Hippuristanol 0.12μM R08 exp489 no dilution day6	0.06	View
5-Fluorouracil 20μM R00 exp2	0.059	View
Tanespimycin 14μM R08 exp527	0.059	View
∆-9-Tetrahydrocannabinol 30μM R08 exp445	0.059	View
Pimelic-diphenylamide-106 5μM R00 exp28	0.058	View
Rotenone 20μM R00 exp34	0.056	View
BI-D1870 3.15μM R02 exp66	0.055	View
Ethanol 0.01 R05 exp234	0.055	View
Etoposide 1μM R00 exp19	0.055	View
CB-5083 0.4μM R08 exp468	0.054	View
AICAR 240μM R05 exp211	0.053	View
FK-506 30μM R07 exp359	0.053	View
Cryptotanshinone 12μM R05 exp227	0.053	View
Cannabidiol 20μM R08 exp466	0.053	View
Dinaciclib 0.007μM R07 exp355	0.053	View
Bisphenol A 100μM R06 exp269	0.052	View
GSK-J4 1.5μM R08 exp441	0.052	View
Doxorubicin 0.02μM R08 exp478	0.051	View

GO Term	Fold Change	Genes
cAMP-dependent protein kinase activity	355.37	PRKAG1,PRKAA1,PRKACG
cyclic nucleotide-dependent protein kinase activity	253.84	PRKAG1,PRKAA1,PRKACG
regulation of TORC1 signaling	27.55	PRKAA1,OTUB1,TBK1,TSC2
transferase complex	7.13	PRKAG1,PRKAA1,WDR26,TRAF2,YPEL5,BCOR,TBK1,MBTD1,RING1,PRKACG
intracellular protein-containing complex	6.07	PRKAG1,PRKAA1,WDR26,TRAF2,YPEL5,BCOR,MBTD1,RING1,PRKACG

GO Term	Fold Change	Genes
protein histidyl modification to diphthamide	442.24	DPH3,DPH1,DPH5,DPH2
peptidyl-histidine modification	236.91	DPH3,DPH5,DPH2
porin activity	221.12	BAK1,VDAC2
metallo-sulfur cluster assembly	59.23	NFU1,IBA57,GLRX5
iron-sulfur cluster assembly	59.23	NFU1,IBA57,GLRX5
positive regulation of response to endoplasmic reticulum stress	47.38	BAK1,UBQLN1,PMAIP1
metal cluster binding	33.00	DPH1,DPH2,NFU1,GLRX5
iron-sulfur cluster binding	33.00	DPH1,DPH2,NFU1,GLRX5
tRNA modification	31.06	DPH3,PUS3,THG1L,ELP6,SEPSECS
tRNA processing	21.94	DPH3,PUS3,THG1L,ELP6,SEPSECS
mitochondrial membrane organization	20.86	BAK1,OMA1,PMAIP1,VDAC2
mitochondrial outer membrane	15.95	MARCHF5,BAK1,STARD7,PMAIP1,VDAC2,THG1L
tRNA metabolic process	14.78	DPH3,PUS3,THG1L,ELP6,SEPSECS
organelle outer membrane	14.05	MARCHF5,BAK1,STARD7,PMAIP1,VDAC2,THG1L
outer membrane	13.94	MARCHF5,BAK1,STARD7,PMAIP1,VDAC2,THG1L
mitochondrial membrane	6.26	MARCHF5,BAK1,STARD7,OMA1,PMAIP1,COA3,VDAC2,THG1L
mitochondrial envelope	5.88	MARCHF5,BAK1,STARD7,OMA1,PMAIP1,COA3,VDAC2,THG1L

Table of Contents

UM0129480 7μM R02 exp85

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results