Diepoxybutane 3μM R07 exp354

DNA crosslinker, carcinogen

Class / Subclass 1: DNA Damage, Repair and Replication / Inter/Intra-strand Crosslinker

1H NMR (CDCl3, 400 MHz): delta 2.95-2.88 (m, 2 H), 2.88-2.81 (m, 2 H), 2.78-2.72 (m, 2 H)
13C NMR (CDCl3, 101 MHz): delta 51.1, 44.2

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
56/39	Scores	View	View	View	View	View

Screen	Correlation	Plot
Nutlin-3A 1.6μM R06 exp294	0.125	View
Olaparib 4μM R08 exp512	0.116	View
Hydroxyurea 100μM R07 exp329	0.08	View
Talazoparib 0.006μM R06 exp299	0.078	View
Rucaparib 6.5μM R08 exp520	0.076	View
Mitomycin-C 0.06μM R08 exp503	0.072	View
Trimetrexate 0.03μM R08 exp535	0.071	View
Quercetin 20μM R08 exp517	0.067	View
Pterostilbene 16μM R02 exp78	0.063	View
Etoposide 1μM R00 exp19	0.062	View
Hippuristanol 0.12μM R08 exp488	0.062	View
Resveratrol 16μM R03 exp147	0.057	View
MK-1775 0.32μM R02 exp75	0.056	View
Lead acetate 2000μM R08 exp497	0.056	View
Lead acetate 2000μM R08 exp498 no dilution day6	0.056	View
Menadione 5μM R06 exp292	0.054	View
Docosahexaenoic-acid 50μM R07 exp356	0.052	View
Sulforaphane 9μM R08 exp525	0.052	View
Ethanol 0.01 R05 exp234	0.052	View
Aphidicolin 0.4μM R08 exp440	0.084	View
5-Fluorouracil 20μM R00 exp2	0.067	View
CI-1040 9.5μM R08 exp472	0.062	View
AICAR 240μM R05 exp211	0.061	View
4-Nitroquinoline-1-oxide 0.5μM R08 exp446	0.059	View
Actinomycin-D 0.001μM R00 exp3	0.058	View
BML-284 0.09μM R08 exp460	0.053	View
AICAR 240μM R03 exp116	0.053	View
Benzoate 20000μM R08 exp456	0.052	View
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	0.052	View

GO Term	Fold Change	Genes
MutSalpha complex binding	212.55	MCM9,ATR
interstrand cross-link repair	93.77	FANCD2,MCM9,ATR,FANCB,FANCC
negative regulation of double-strand break repair	65.96	OTUB1,FANCB,MAD2L2
negative regulation of DNA repair	61.71	OTUB1,FANCB,MAD2L2
regulation of double-strand break repair	25.92	OTUB1,ATR,FANCB,MORF4L1,MAD2L2
ATP-dependent activity, acting on DNA	22.37	BRIP1,DDX3X,CHD1,MCM9
double-strand break repair	19.03	BRIP1,FANCD2,MCM9,FANCB,MORF4L1,MAD2L2
regulation of DNA repair	15.78	OTUB1,ATR,FANCB,MORF4L1,MAD2L2
DNA recombination	14.23	BRIP1,FANCD2,MCM9,FANCB,MORF4L1
DNA repair	12.18	OTUB1,BRIP1,FANCD2,MCM9,ATR,FANCB,MORF4L1,FANCC,MAD2L2
DNA damage response	8.04	OTUB1,BRIP1,FANCD2,MCM9,ATR,FANCB,MORF4L1,FANCC,MAD2L2
DNA metabolic process	8.02	OTUB1,BRIP1,FANCD2,MCM9,ATR,FANCB,MORF4L1,FANCC,MAD2L2

GO Term	Fold Change	Genes
RecQ family helicase-topoisomerase III complex	552.63	RMI2,RMI1,BLM
resolution of recombination intermediates	331.58	RMI2,RMI1,BLM
cellular senescence	36.84	PML,TP53,SMC5,SMC6
replication fork	34.54	BLM,TP53,CARM1,POLA1
PML body	31.58	PML,BLM,TP53,TOP3A,SMC5,SMC6
single-stranded DNA binding	29.34	BLM,TP53,TOP3A,SMC5,POLA1,SMC6
double-strand break repair via homologous recombination	28.58	RMI2,RMI1,BLM,TOP3A,SMC5,SMC6
recombinational repair	27.86	RMI2,RMI1,BLM,TOP3A,SMC5,SMC6
double-strand break repair	22.00	RMI2,RMI1,BLM,TP53,TOP3A,SMC5,POLA1,SMC6
chromosome, telomeric region	18.06	PML,BLM,SMC5,SMC6,PPP1R10
DNA replication	17.00	RMI2,RMI1,SLFN11,BLM,CARM1,POLA1
DNA recombination	14.80	RMI2,RMI1,BLM,TOP3A,SMC5,SMC6
negative regulation of cell cycle process	12.75	PML,SLFN11,BLM,TP53,NSUN2,PPP1R10
DNA repair	10.56	RMI2,RMI1,BLM,TP53,TOP3A,SMC5,PWWP3A,POLA1,SMC6
DNA damage response	9.29	RMI2,RMI1,PML,SLFN11,BLM,TP53,TOP3A,SMC5,PWWP3A,POLA1,BAK1,SMC6
regulation of DNA metabolic process	8.70	RMI2,PML,SLFN11,BLM,TP53,SMC5,SMC6,PPP1R10
DNA metabolic process	8.49	RMI2,RMI1,SLFN11,BLM,TP53,TOP3A,CARM1,SMC5,PWWP3A,POLA1,SMC6
nuclear body	7.48	RMI2,RMI1,PML,BLM,TP53,TOP3A,SMC5,CDK13,SMC6,PPP1R10,PNISR
regulation of cell cycle process	7.19	RMI2,PML,SLFN11,BLM,TP53,SMC5,NSUN2,SMC6,PPP1R10