NSF
Gene Information
- Official Symbol: NSF
- Official Name: N-ethylmaleimide sensitive factor, vesicle fusing ATPase
- Aliases and Previous Symbols: N/A
- Entrez ID: 4905
- UniProt: P46459
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: N/A
- UniProt Summary: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity). {ECO:0000250}.
Pfam Domains GO Terms
Pfam Domains
| CDC48 2 |
| CDC48 N |
| AAA 2 |
| AAA |
GO Terms
| Golgi vesicle docking |
| SNARE complex disassembly |
| positive regulation of receptor recycling |
| plasma membrane fusion |
| ATPase activity, coupled |
| regulation of receptor recycling |
| syntaxin-1 binding |
| ionotropic glutamate receptor binding |
| Golgi to plasma membrane protein transport |
| Golgi stack |
| dendritic shaft |
| establishment of protein localization to plasma membrane |
| Golgi to plasma membrane transport |
| vesicle docking |
| vesicle targeting, rough ER to cis-Golgi |
| COPII vesicle coating |
| SNARE binding |
| vesicle coating |
| COPII-coated vesicle budding |
| vesicle targeting, to, from or within Golgi |
| Golgi vesicle budding |
| retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum |
| PDZ domain binding |
| vesicle targeting |
| vesicle-mediated transport to the plasma membrane |
| vesicle budding from membrane |
| post-Golgi vesicle-mediated transport |
| membrane fusion |
| cellular protein complex disassembly |
| Rab GTPase binding |
| potassium ion transport |
| organelle localization by membrane tethering |
| establishment of vesicle localization |
| membrane docking |
| protein localization to plasma membrane |
| vesicle localization |
| endoplasmic reticulum to Golgi vesicle-mediated transport |
| positive regulation of protein catabolic process |
| regulation of exocytosis |
| protein localization to cell periphery |
| ATPase activity |
| protein-containing complex disassembly |
| postsynaptic density |
| establishment of protein localization to membrane |
| protein-containing complex binding |
| vesicle organization |
| lysosomal membrane |
| establishment of organelle localization |
| Golgi vesicle transport |
| regulation of protein catabolic process |
| cellular component disassembly |
| monovalent inorganic cation transport |
| positive regulation of catabolic process |
| protein kinase binding |
| protein localization to membrane |
| regulation of vesicle-mediated transport |
| organelle localization |
| Golgi membrane |
| metal ion transport |
| regulation of secretion by cell |
| exocytosis |
| regulation of secretion |
| cation transport |
| membrane organization |
| regulation of catabolic process |
| intracellular protein transport |
| secretion by cell |
| export from cell |
| secretion |
| ion transport |
| ATP binding |
| protein transport |
| intracellular transport |
| peptide transport |
| protein-containing complex assembly |
| amide transport |
| cellular protein localization |
| cellular macromolecule localization |
| establishment of protein localization |
| positive regulation of protein metabolic process |
| positive regulation of signaling |
| establishment of localization in cell |
| nitrogen compound transport |
| protein-containing complex subunit organization |
| regulation of transport |
| vesicle-mediated transport |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
| Screen | Score |
|---|---|
| Tanespimycin 14μM R08 exp527 | -2.49 |
| Vinblastine 0.015μM R05 exp208 | -2.16 |
| Deferoxamine 11μM R07 exp350 | -1.84 |
| Benzoate 20000μM R08 exp456 | -1.82 |
| UMK57 1μM R01 exp59 | -1.74 |
| SGC0649 7μM R03 exp150 | 1.97 |
| LB-100 2μM R05 exp210 | 2.37 |
Most Correlated Genes in Chemogenomics
| Gene | Correlation |
|---|---|
| HGC6.3 | 0.576 |
| TFIP11 | 0.529 |
| IK | 0.524 |
| UTP3 | 0.504 |
| DOCK6 | 0.501 |
| PSMB4 | 0.499 |
| COPG1 | 0.494 |
| RPS2 | 0.492 |
| COPA | 0.487 |
| POLR2J3 | 0.486 |
| ATP6V1B2 | 0.484 |
| CCNA2 | 0.484 |
| VPS28 | 0.482 |
| TCEB3CL2 | 0.475 |
| TYMS | 0.475 |
| TSPY8 | 0.473 |
| TCEB3C | 0.472 |
| TWISTNB | 0.469 |
| CHD4 | 0.468 |
| ZNF830 | 0.466 |
| TIMELESS | 0.466 |
| PSMB3 | 0.463 |
| GOSR2 | 0.462 |
| TINF2 | 0.462 |
| CBWD3 | 0.462 |
| RANBP2 | 0.462 |
| EXOSC6 | 0.461 |
| CBWD6 | 0.459 |
| EXOSC3 | 0.458 |
| TCEB3CL | 0.455 |
| CBWD5 | 0.455 |
| NARFL | 0.453 |
| NUPL1 | 0.451 |
| TICRR | 0.449 |
| C14orf93 | 0.447 |
| CACTIN | 0.446 |
| RPS12 | 0.446 |
| COPZ1 | 0.446 |
| ACSF2 | 0.444 |
| PSMC2 | 0.443 |
| NPIPB5 | 0.441 |
| C1QTNF9B | 0.441 |
| CCDC64B | 0.44 |
| ATP6V1C1 | 0.44 |
| ANKLE1 | 0.44 |
| RNPC3 | 0.44 |
| TRAPPC11 | 0.44 |
| TP53TG3 | 0.439 |
| TP53TG3B | 0.439 |
| EXOSC9 | 0.438 |
| TP53TG3C | 0.438 |
| NOP14 | 0.437 |
| AKIRIN2 | 0.437 |
| SCGB1C1 | 0.435 |
| PDCD7 | 0.435 |
| MT1E | 0.435 |
| CDC6 | 0.435 |
| NFATC4 | 0.434 |
| SF3A1 | 0.433 |
| SULT2B1 | 0.433 |
| PSMB2 | 0.431 |
| SLC4A1 | 0.43 |
| DISP2 | 0.43 |
| RPL6 | 0.428 |
| PSMA6 | 0.428 |
| PDCD11 | 0.428 |
| RPL35 | 0.427 |
| CTAGE9 | 0.427 |
| PSMB1 | 0.425 |
| RPL14 | 0.421 |
| TBC1D3H | 0.42 |
| ZFAND5 | 0.418 |
| RPL30 | 0.417 |
| PSMC5 | 0.416 |
| TBC1D3G | 0.416 |
| FAM231C | 0.415 |
| FAM231A | 0.415 |
| DDX47 | 0.415 |
| SNIP1 | 0.414 |
| EIF3G | 0.414 |
| SUPT5H | 0.414 |
| ORC6 | 0.413 |
| KCTD17 | 0.412 |
| FOXD4L2 | 0.411 |
| MEPCE | 0.411 |
| RPLP1 | 0.41 |
| CTAGE4 | 0.41 |
| SETD1A | 0.41 |
| KIF3C | 0.41 |
| NOL9 | 0.41 |
| TBC1D3F | 0.41 |
| XAB2 | 0.409 |
| NUP153 | 0.409 |
| DDTL | 0.408 |
| CDH24 | 0.408 |
| AAMP | 0.408 |
| WDR75 | 0.408 |
| RPS5 | 0.406 |
| KRT78 | 0.406 |
| MYO1H | 0.405 |
| RAD51 | 0.405 |
| CCDC94 | 0.405 |
| VASN | 0.404 |
| FAM25B | 0.403 |
| AKAP1 | 0.403 |
| COL2A1 | 0.402 |
| NUP160 | 0.402 |
| CHMP4B | 0.401 |
| GAR1 | 0.401 |
| POLR2D | 0.401 |
| CMYA5 | 0.4 |
| RRP1 | 0.4 |
| HIVEP3 | 0.4 |
| CDC45 | 0.4 |
| AKT1S1 | 0.4 |
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 735/739
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 1/1 |
| 909776.0 | 1/1 |
| bile duct | 27/28 |
| blood | 28/28 |
| bone | 26/26 |
| breast | 33/33 |
| central nervous system | 56/56 |
| cervix | 4/4 |
| colorectal | 17/17 |
| esophagus | 13/13 |
| fibroblast | 1/1 |
| gastric | 16/16 |
| kidney | 21/21 |
| liver | 20/20 |
| lung | 74/75 |
| lymphocyte | 16/16 |
| ovary | 26/26 |
| pancreas | 24/24 |
| peripheral nervous system | 16/16 |
| plasma cell | 15/15 |
| prostate | 1/1 |
| skin | 24/24 |
| soft tissue | 9/9 |
| thyroid | 2/2 |
| upper aerodigestive | 22/22 |
| urinary tract | 28/29 |
| uterus | 5/5 |
Essentiality in NALM6
- Essentiality Rank: 336
- Expression level (log2 read counts): 6.57
Expression Distribution
NSF Expression in NALM6 Cells: 6.57
