VPS28
Gene Information
- Official Symbol: VPS28
- Official Name: VPS28 subunit of ESCRT-I
- Aliases and Previous Symbols: N/A
- Entrez ID: 51160
- UniProt: Q9UK41
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013].
- UniProt Summary: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. {ECO:0000269|PubMed:11916981}.
Pfam Domains GO Terms
Pfam Domains
| VPS28 |
GO Terms
| positive regulation of ubiquitin-dependent endocytosis |
| regulation of ubiquitin-dependent endocytosis |
| protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway |
| late endosome to vacuole transport via multivesicular body sorting pathway |
| ESCRT I complex |
| late endosome to vacuole transport |
| viral budding via host ESCRT complex |
| ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway |
| viral budding |
| endosome transport via multivesicular body sorting pathway |
| multivesicular body assembly |
| multivesicular body organization |
| multivesicular body sorting pathway |
| virion assembly |
| establishment of protein localization to vacuole |
| intracellular transport of virus |
| host cell |
| transport of virus |
| protein localization to vacuole |
| multi-organism localization |
| multi-organism transport |
| negative regulation of protein ubiquitination |
| endosome organization |
| ubiquitin binding |
| negative regulation of protein modification by small protein conjugation or removal |
| positive regulation of endocytosis |
| late endosome membrane |
| vacuolar transport |
| macroautophagy |
| regulation of protein ubiquitination |
| viral life cycle |
| regulation of endocytosis |
| endosome membrane |
| positive regulation of protein catabolic process |
| regulation of protein modification by small protein conjugation or removal |
| endosomal transport |
| early endosome |
| autophagy |
| process utilizing autophagic mechanism |
| protein-containing complex binding |
| endosome |
| vesicle organization |
| positive regulation of cellular protein localization |
| regulation of protein catabolic process |
| endomembrane system organization |
| positive regulation of protein transport |
| establishment of protein localization to organelle |
| positive regulation of catabolic process |
| positive regulation of establishment of protein localization |
| ubiquitin-dependent protein catabolic process |
| modification-dependent protein catabolic process |
| regulation of cellular protein localization |
| modification-dependent macromolecule catabolic process |
| regulation of vesicle-mediated transport |
| proteolysis involved in cellular protein catabolic process |
| negative regulation of protein modification process |
| cellular protein catabolic process |
| protein catabolic process |
| viral process |
| regulation of protein transport |
| protein localization to organelle |
| regulation of peptide transport |
| regulation of establishment of protein localization |
| organelle assembly |
| symbiotic process |
| interspecies interaction between organisms |
| cellular macromolecule catabolic process |
| regulation of cellular localization |
| positive regulation of transport |
| regulation of catabolic process |
| intracellular protein transport |
| regulation of protein localization |
| negative regulation of cellular protein metabolic process |
| macromolecule catabolic process |
| organonitrogen compound catabolic process |
| negative regulation of protein metabolic process |
| positive regulation of cellular component organization |
| proteolysis |
| protein transport |
| intracellular transport |
| peptide transport |
| amide transport |
| cellular protein localization |
| cellular macromolecule localization |
| establishment of protein localization |
| positive regulation of protein metabolic process |
| organic substance catabolic process |
| cellular catabolic process |
| establishment of localization in cell |
| regulation of protein modification process |
| nitrogen compound transport |
| regulation of transport |
| vesicle-mediated transport |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
| Screen | Score |
|---|---|
| Nocodazole 0.2μM R00 exp24 | -2.86 |
| Bortezomib 0.05μM R00 exp7 | -2.33 |
| BI-6727 0.04μM R03 exp98 | -2.31 |
| GSK461364A 0.1μM R03 exp126 | -2.19 |
| Rotenone 20μM R00 exp34 | -2.17 |
| Rotenone 2μM R00 exp33 | -2.08 |
| Vinblastine 0.2μM R03 exp108 | -2.02 |
| Actinomycin-D 0.001μM R00 exp3 | -1.85 |
| Etoposide 1μM R00 exp19 | -1.84 |
| Oligomycin-A 20μM R00 exp26 | -1.8 |
Most Correlated Genes in Chemogenomics
| Gene | Correlation |
|---|---|
| PSMB4 | 0.59 |
| TCEB3C | 0.566 |
| POLR2J3 | 0.557 |
| COPG1 | 0.555 |
| TCEB3CL | 0.553 |
| TCEB3CL2 | 0.547 |
| RPL35 | 0.544 |
| IK | 0.532 |
| EXOSC3 | 0.521 |
| ATP6V1B2 | 0.519 |
| AATF | 0.518 |
| EXOSC9 | 0.514 |
| TRAPPC11 | 0.513 |
| TYMS | 0.512 |
| GOLGA6L1 | 0.508 |
| UTP3 | 0.506 |
| RRP7A | 0.495 |
| AQR | 0.493 |
| HGC6.3 | 0.491 |
| COL2A1 | 0.49 |
| CHAF1A | 0.489 |
| CCNA2 | 0.488 |
| SUPT5H | 0.483 |
| NSF | 0.482 |
| DHX38 | 0.481 |
| ZNF830 | 0.477 |
| TBC1D3H | 0.476 |
| CDH24 | 0.476 |
| COX7A2L | 0.473 |
| CEP192 | 0.469 |
| TBC1D3F | 0.468 |
| CHD4 | 0.467 |
| PSMB2 | 0.467 |
| SCGB1C1 | 0.467 |
| C1QTNF9B | 0.462 |
| VPS53 | 0.46 |
| PSMC2 | 0.459 |
| RNPC3 | 0.458 |
| CTAGE9 | 0.458 |
| C14orf93 | 0.457 |
| PRPF19 | 0.457 |
| ATP6AP1 | 0.456 |
| NUP160 | 0.456 |
| CTAGE4 | 0.455 |
| PSMA7 | 0.454 |
| MTCH2 | 0.453 |
| PSMA6 | 0.451 |
| TSG101 | 0.45 |
| INTS5 | 0.45 |
| TSPY8 | 0.45 |
| TBC1D3G | 0.445 |
| DOCK6 | 0.445 |
| DTL | 0.445 |
| SH3BP1 | 0.441 |
| RPL8 | 0.44 |
| TRAPPC5 | 0.439 |
| NARFL | 0.437 |
| NPIPB5 | 0.437 |
| POLR2D | 0.436 |
| AKT1S1 | 0.436 |
| CDCA3 | 0.436 |
| TP53TG3B | 0.435 |
| TP53TG3C | 0.435 |
| TP53TG3 | 0.435 |
| TICRR | 0.432 |
| EXOSC8 | 0.431 |
| SNIP1 | 0.43 |
| IL2RB | 0.43 |
| FAM20A | 0.429 |
| PAN3 | 0.429 |
| CACTIN | 0.429 |
| TP53TG3D | 0.428 |
| CBWD3 | 0.428 |
| EXOSC6 | 0.428 |
| RPS2 | 0.428 |
| MADCAM1 | 0.426 |
| RPL6 | 0.426 |
| PNO1 | 0.426 |
| TBC1D3 | 0.425 |
| RPS19BP1 | 0.425 |
| NUPL1 | 0.424 |
| AAMP | 0.423 |
| KRT78 | 0.423 |
| GOLGA8O | 0.423 |
| EBNA1BP2 | 0.42 |
| POLE | 0.419 |
| EIF4A3 | 0.419 |
| AKAP1 | 0.418 |
| DISP2 | 0.418 |
| VPS51 | 0.417 |
| VASN | 0.417 |
| RPS12 | 0.417 |
| UTP20 | 0.417 |
| CBWD6 | 0.417 |
| SLC4A1 | 0.417 |
| ORC1 | 0.416 |
| RPL30 | 0.416 |
| ATP6V1E1 | 0.415 |
| PRIM1 | 0.415 |
| GAR1 | 0.414 |
| POLR1A | 0.414 |
| SNRPA1 | 0.414 |
| RPL34 | 0.412 |
| GOLGA6L4 | 0.412 |
| PSMD7 | 0.411 |
| TIMELESS | 0.411 |
| CBWD5 | 0.41 |
| PSMB3 | 0.41 |
| RPL37A | 0.41 |
| SULT1A4 | 0.409 |
| SULT1A3 | 0.409 |
| FAM231A | 0.408 |
| RANBP2 | 0.408 |
| NOL10 | 0.408 |
| TFIP11 | 0.408 |
| FAM231C | 0.408 |
| GOLGA6L3 | 0.408 |
| NFYA | 0.408 |
| RRN3 | 0.406 |
| DDTL | 0.406 |
| CDT1 | 0.406 |
| EIF3G | 0.406 |
| CCDC84 | 0.405 |
| SRSF3 | 0.405 |
| TBC1D3I | 0.404 |
| TBC1D3C | 0.404 |
| RANGAP1 | 0.403 |
| TBC1D3E | 0.403 |
| USP35 | 0.403 |
| TBC1D3L | 0.402 |
| TBC1D3K | 0.402 |
| WDR75 | 0.402 |
| TINF2 | 0.402 |
| POLR2A | 0.401 |
| ATP6V0C | 0.4 |
| TWISTNB | 0.4 |
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 401/739
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 1/1 |
| 909776.0 | 0/1 |
| bile duct | 12/28 |
| blood | 15/28 |
| bone | 19/26 |
| breast | 17/33 |
| central nervous system | 30/56 |
| cervix | 2/4 |
| colorectal | 12/17 |
| esophagus | 5/13 |
| fibroblast | 0/1 |
| gastric | 7/16 |
| kidney | 11/21 |
| liver | 13/20 |
| lung | 40/75 |
| lymphocyte | 9/16 |
| ovary | 15/26 |
| pancreas | 9/24 |
| peripheral nervous system | 7/16 |
| plasma cell | 9/15 |
| prostate | 1/1 |
| skin | 15/24 |
| soft tissue | 7/9 |
| thyroid | 1/2 |
| upper aerodigestive | 5/22 |
| urinary tract | 15/29 |
| uterus | 4/5 |
Essentiality in NALM6
- Essentiality Rank: 561
- Expression level (log2 read counts): 4.84
Expression Distribution
VPS28 Expression in NALM6 Cells: 4.84
