Table of Contents
Colchicine 0.009μM R05 exp215
Mechanism of Action
Inhibits microtubule polymerization
- Class / Subclass 1: Cell Cycle / Microtubule Poison
- Class / Subclass 2: Organelle Function / Cytoskeletal Inhibitor
Technical Notes
Protein References
- PubChem Name: Colchicine
- Synonyms: N/A
- CAS #: 64-86-8
- PubChem CID: 6167
- IUPAC: N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[a]heptalen-7-yl]acetamide
- INCHI Name: InChI=1S/C22H25NO6/c1-12(24)23-16-8-6-13-10-19(27-3)21(28-4)22(29-5)20(13)14-7-9-18(26-2)17(25)11-15(14)16/h7,9-11,16H,6,8H2,1-5H3,(H,23,24)/t16-/m0/s1
- INCHI Key: IAKHMKGGTNLKSZ-INIZCTEOSA-N
- Molecular Weight: 399.4
- Canonical SMILES: CC(=O)NC1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
- Isomeric SMILES: CC(=O)N[C@H]1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
- Molecular Formula: C22H25NO6
Protein Supplier
- Supplier Name: Sigma-Aldrich
- Catalog #: C9754
- Lot #: N/A
Protein Characterization
- HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C22H25NO6 400.17546; found 400.17564
Dose Response Curve
- Platform ID: Colchicine
- Min: -2.2590; Max: 86.4027
| IC | Concentration (µM) |
|---|---|
| IC10 | N/A |
| IC20 | 0.0071 |
| IC30 | 0.0091 |
| IC40 | 0.0111 |
| IC50 | 0.0133 |
| IC60 | N/A |
| IC70 | N/A |
| IC80 | N/A |
| IC90 | N/A |
Screen Summary
- Round: 05
- Dose: 9nM
- Days of incubation: 8
- Doublings: 5.5
- Numbers of reads: 19549288
Screen Results
| Sensitive/Resistant hits (FDR<0.05) | CRANKS | Score Plot | Top 30 Genes | Screen Similarity | Top 30 Sensitive GO terms | Top 30 Resistant GO terms |
|---|---|---|---|---|---|---|
| 58/26 | Scores |
Colchicine 0.009μM R05 exp215
Colchicine 0.009μM R05 exp215 vs Demecolcine 0.03μM R05 exp228
Colchicine 0.009μM R05 exp215 vs Combretastatin A4 0.002 to 0.003μM day4 R04 exp179
Colchicine 0.009μM R05 exp215 vs Vincristine 0.0005μM R04 exp190
Colchicine 0.009μM R05 exp215 vs Mdivi-1 15μM R05 exp217
Colchicine 0.009μM R05 exp215 vs UMK57 1μM R01 exp59
Colchicine 0.009μM R05 exp215 vs S-trityl-L-cysteine 0.5μM R05 exp243
Colchicine 0.009μM R05 exp215 vs Quercetin 20μM R08 exp517
Colchicine 0.009μM R05 exp215 vs Ixabepilone 0.004 to 0.005μM on day4 R04 exp184
Colchicine 0.009μM R05 exp215 vs Vinblastine 0.002μM R01 exp60
Colchicine 0.009μM R05 exp215 vs UM131593 0.2μM R03 exp106
Colchicine 0.009μM R05 exp215 vs Taxol 0.004μM R03 exp103
Colchicine 0.009μM R05 exp215 vs Epothilone-B 0.0015μM R05 exp231
BML-284 0.09μM R08 exp460 vs Colchicine 0.009μM R05 exp215
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312 vs Colchicine 0.009μM R05 exp215
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311 vs Colchicine 0.009μM R05 exp215
BI-6727 0.0125μM R03 exp97 vs Colchicine 0.009μM R05 exp215
2-Methoxyestradiol 0.55μM R07 exp310 vs Colchicine 0.009μM R05 exp215
