CDT1

Gene Information

Official Symbol: CDT1
Official Name: chromatin licensing and DNA replication factor 1
Aliases and Previous Symbols: N/A
Entrez ID: 81620
UniProt: Q9H211
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein. [provided by RefSeq, Mar 2011].
UniProt Summary: Required for both DNA replication and mitosis (PubMed:11125146, PubMed:22581055, PubMed:21856198, PubMed:14993212). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre- RC)(PubMed:14672932). Required also for mitose by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene (By similarity). {ECO:0000250|UniProtKB:Q8R4E9, ECO:0000269|PubMed:11125146, ECO:0000269|PubMed:14672932, ECO:0000269|PubMed:14993212, ECO:0000269|PubMed:21856198, ECO:0000269|PubMed:22581055}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
SNS-032 25μM R07 exp401	-2.75
6-Thio-2-deoxyguanosine 2μM R06 exp259	-2.24
Etoposide 0.3μM R05 exp199	-2.1
Nutlin-3A 1.6μM R06 exp294	-2.09
MS023 2μM R03 exp134	-1.93
Nocodazole 0.2μM R00 exp24	-1.91
Palbociclib 1μM R07 exp382	-1.89
Atovaquone 15μM R08 exp451	-1.86
Vinblastine 0.015μM R05 exp208	-1.86
Dinaciclib 0.007μM R07 exp355	-1.78
IU1-47 25μM R04 exp182	-1.72
Pimelic-diphenylamide-106 5μM R00 exp28	-1.7
Rapamycin 10μM R00 exp30	1.7
BI-2536 0.001μM R01 exp41	1.73
CCCP 0.1μM R00 exp10	1.77
Dihydrosphingosine 8μM R08 exp476	1.79
Tenofovir 10μM R07 exp405	1.79
Epothilone-D 0.004 to 0.006μM on day4 R05 exp232	1.82
Lead acetate 990μM R08 exp496	1.85
Rifampicin 10μM R00 exp32	1.86
ABT-702 0.1μM R06 exp260	1.93
CVT-10216 0.1μM R06 exp278	2.11
GSK-LSD1 10μM R03 exp123	2.18
GW501516 25μM R06 exp285	2.53
Metformin 100 to 150μM on day4 R04 exp170	3.05
Nocodazole 0.02μM R00 exp23	3.18

Gene	Correlation
GOLGA6L1	0.572
PSMB4	0.521
NOP16	0.479
RFC1	0.47
ZFAND5	0.469
RPL35	0.468
PSMD11	0.466
RPS2	0.465
CHD4	0.46
DDTL	0.456
CCNA2	0.456
EXOSC5	0.453
CHMP4B	0.452
PSMA6	0.451
RRM1	0.447
RPS12	0.443
XYLT2	0.443
ZNF830	0.443
IK	0.443
TBC1D3H	0.441
EXOSC9	0.438
MT1E	0.437
ORC6	0.436
PAN2	0.435
IGFN1	0.434
PSMD1	0.433
GOSR2	0.432
UTP3	0.432
PSMA1	0.43
NPIPB5	0.43
RRN3	0.428
DHX38	0.427
TBC1D3G	0.427
DTL	0.425
PRPF19	0.425
CDC73	0.424
SF3A3	0.423
SKIV2L2	0.423
PPM1D	0.423
POLR2H	0.42
EXOSC3	0.416
RPS19	0.416
COL2A1	0.414
PSMC2	0.413
PSMB1	0.413
NARFL	0.413
PAN3	0.411
TCEB3CL2	0.411
DDX56	0.411
SUPT6H	0.41
NUPL1	0.41
PSMC1	0.41
CHMP2A	0.41
SMTN	0.409
RPS19BP1	0.408
TCEB3C	0.408
CBWD3	0.407
TFIP11	0.407
CTAGE4	0.407
PCF11	0.407
CCDC116	0.407
VPS28	0.406
TBC1D3C	0.405
PAF1	0.405
MBTD1	0.405
TSPY8	0.403
PSMB3	0.403
CBWD5	0.402
SCAF1	0.402
ARCN1	0.401
SMG1	0.4
TBC1D3F	0.4

Global Fraction of Cell Lines Where Essential: 712/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	27/28
blood	28/28
bone	23/26
breast	31/33
central nervous system	54/56
cervix	4/4
colorectal	15/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	20/21
liver	20/20
lung	74/75
lymphocyte	16/16
ovary	25/26
pancreas	23/24
peripheral nervous system	16/16
plasma cell	13/15
prostate	1/1
skin	24/24
soft tissue	8/9
thyroid	2/2
upper aerodigestive	21/22
urinary tract	28/29
uterus	4/5

Essentiality in NALM6

Essentiality Rank: 969
Expression level (log2 read counts): 6.49

Expression Distribution

deactivation of mitotic spindle assembly checkpoint
negative regulation of spindle checkpoint
negative regulation of protein localization to kinetochore
regulation of mediator complex assembly
positive regulation of mediator complex assembly
regulation of DNA replication origin binding
DNA replication preinitiation complex assembly
regulation of protein localization to kinetochore
positive regulation of protein localization to kinetochore
response to sorbitol
regulation of DNA-dependent DNA replication initiation
negative regulation of cell cycle checkpoint
positive regulation of mitotic metaphase/anaphase transition
positive regulation of mitotic sister chromatid separation
positive regulation of metaphase/anaphase transition of cell cycle
attachment of mitotic spindle microtubules to kinetochore
positive regulation of chromosome separation
positive regulation of DNA-dependent DNA replication
positive regulation of chromatin binding
regulation of mitotic cell cycle spindle assembly checkpoint
regulation of mitotic spindle checkpoint
regulation of spindle checkpoint
DNA replication checkpoint
regulation of nuclear cell cycle DNA replication
positive regulation of mitotic sister chromatid segregation
kinetochore organization
DNA polymerase binding
attachment of spindle microtubules to kinetochore
regulation of chromatin binding
regulation of transcription involved in G1/S transition of mitotic cell cycle
positive regulation of chromosome segregation
regulation of cell cycle checkpoint
positive regulation of DNA replication
nuclear DNA replication
mitotic metaphase plate congression
cell cycle DNA replication
regulation of mitotic metaphase/anaphase transition
regulation of metaphase/anaphase transition of cell cycle
positive regulation of mitotic nuclear division
regulation of DNA-dependent DNA replication