RFK

Entrez Summary: N/A
UniProt Summary: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. {ECO:0000269|PubMed:19641494}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Ribavirin 10 to 15μM on day4 R04 exp160	-2.04
Momordin-Ic 10μM R08 exp506	-1.81
Metformin 40μM R03 exp132	-1.76
Caffeine 2600μM R08 exp463	-1.73
All-trans-Retinoic-Acid 40μM R07 exp334	1.7
UM0125461 0.74μM R02 exp84	1.72
6-Thio-2-deoxyguanosine 2μM R06 exp259	1.74
GSK-J4 1.5μM R08 exp441	1.81
BI-6727 0.04μM R03 exp98	1.82
Calcium Ionophore 0.4μM R07 exp342	1.87
Rapamycin 10μM R00 exp30	1.88
Tanespimycin 14μM R08 exp527	1.88
Cryptotanshinone 12μM R05 exp227	1.89
Cannabidiol 20μM R08 exp466	1.93
ONC212 0.15μM R08 exp513	1.94
Nocodazole 0.2μM R00 exp24	1.95
MLN-4924 2μM R00 exp22	2.31
RK-33 8μM R08 exp518	2.36
Atovaquone 15μM R08 exp451	2.41
Q15 2.7μM R02 exp79	2.43
Oligomycin-A 2μM R00 exp25	2.67
UM0129480 7μM R02 exp85	2.93
Artemisinin 50μM R08 exp450	3.5
Mitomycin-C 0.06μM R08 exp503	6.24

Global Fraction of Cell Lines Where Essential: 41/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	2/28
blood	7/28
bone	0/26
breast	4/33
central nervous system	0/56
cervix	1/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	4/21
liver	0/20
lung	4/75
lymphocyte	3/16
ovary	2/26
pancreas	3/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

FMN biosynthetic process
FMN metabolic process
riboflavin kinase activity
riboflavin biosynthetic process
flavin-containing compound biosynthetic process
positive regulation of NAD(P)H oxidase activity
riboflavin metabolic process
flavin-containing compound metabolic process
water-soluble vitamin biosynthetic process
regulation of NAD(P)H oxidase activity
vitamin biosynthetic process
ribonucleoside monophosphate biosynthetic process
nucleoside monophosphate biosynthetic process
ribonucleoside monophosphate metabolic process
positive regulation of oxidoreductase activity
nucleoside monophosphate metabolic process
water-soluble vitamin metabolic process
regulation of oxidoreductase activity
reactive oxygen species metabolic process
vitamin metabolic process
coenzyme biosynthetic process
ribonucleotide biosynthetic process
ribose phosphate biosynthetic process
cofactor biosynthetic process
nucleotide biosynthetic process
nucleoside phosphate biosynthetic process
coenzyme metabolic process
ribonucleotide metabolic process
ribose phosphate metabolic process
cofactor metabolic process
nucleotide metabolic process
nucleoside phosphate metabolic process
drug metabolic process
organophosphate biosynthetic process
nucleobase-containing small molecule metabolic process
small molecule biosynthetic process
carbohydrate derivative biosynthetic process
organophosphate metabolic process
apoptotic process
carbohydrate derivative metabolic process