Amiloride 100μM R08 exp447

Mechanism of Action

Inhibits epithelial sodium channel (ENaC) and other Na+/H+ channels, also inhibits urokinase-type plasminogen activator (uPA)

  • Class / Subclass 1: Signal Transduction / Ion Channel Inhibitor

Technical Notes

Protein References

  • PubChem Name: Amiloride hydrochloride
  • Synonyms: MK-870 hydrochloride
  • CAS #: 2016-88-8
  • PubChem CID: 16230
  • IUPAC: 3,5-diamino-6-chloro-N-(diaminomethylidene)pyrazine-2-carboxamide;hydrochloride
  • INCHI Name: InChI=1S/C6H8ClN7O.ClH/c7-2-4(9)13-3(8)1(12-2)5(15)14-6(10)11;/h(H4,8,9,13)(H4,10,11,14,15);1H
  • INCHI Key: ACHKKGDWZVCSNH-UHFFFAOYSA-N
  • Molecular Weight: 266.09
  • Canonical SMILES: C1(=C(N=C(C(=N1)Cl)N)N)C(=O)N=C(N)N.Cl
  • Isomeric SMILES: N/A
  • Molecular Formula: C6H9Cl2N7O

Protein Supplier

  • Supplier Name: Med Chem Express
  • Catalog #: HY-B0285A
  • Lot #: 14566

Protein Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C6H8ClN7O 230.05516; found 230.05522

Dose Response Curve

  • Platform ID: Amiloride
  • Min: -0.7865; Max: 67.7240





IC Concentration (µM)
IC10 3.7720
IC20 16.2300
IC30 42.8000
IC40 94.7900
IC50 196.6000
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

  • Round: 08
  • Dose: 100µM
  • Days of incubation: 8
  • Doublings: 2.5
  • Numbers of reads: 18416066

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
10/36ScoresViewViewViewViewView

  • Last modified: 2026/01/07 22:37
  • by 127.0.0.1