Table of Contents

TRAIL 50ng/ml R00 exp36

TRAIL 50ng/ml R00 exp36

Mechanism of Action

TNF-related apoptosis-inducing ligand, TNF superfamily member, induces extrinsic apoptosis

Class / Subclass 1: Signal Transduction / Other Receptor Agonist

Technical Notes

Protein References

PubChem Name: N/A
Synonyms: N/A
CAS #: N/A
PubChem CID: N/A
IUPAC: N/A
INCHI Name: N/A
INCHI Key: N/A
Molecular Weight: 19.6kDa
Canonical SMILES: N/A
Isomeric SMILES: N/A
Molecular Formula: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE

Protein Supplier

Supplier Name: Peprotech
Catalog #: 310-04
Lot #: N/A

Protein Characterization

Characterization data not available.

Dose Response Curve

Platform ID: Trail
Min: -9.0552; Max: 7.2598

IC	Concentration (µM)
IC10	N/A
IC20	N/A
IC30	N/A
IC40	N/A
IC50	N/A
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 00
Dose: 50ng/mL
Days of incubation: 8
Doublings: 2.1
Numbers of reads: 13298540

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
60/122	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
CNOT2	-4.56	<0.01
NPRL3	-4.03	<0.01
DEPDC5	-3.59	<0.01
ZBTB1	-3.44	<0.01
SZT2	-3.39	<0.01
RNF19B	-3.35	<0.01
BCL11A	-3.26	<0.01
MBD3	-3.20	<0.01
CNOT8	-3.15	<0.01
GLRX3	-3.12	<0.01
ARID4B	-3.09	<0.01
MIDN	-3.03	<0.01
UBE2D3	-3.02	<0.01
PRKAG1	-3.02	<0.01
PCBP2	-2.97	<0.01
PDS5B	-2.93	<0.01
UBE2N	-2.88	<0.01
NPRL2	-2.83	<0.01
RB1CC1	-2.79	<0.01
WDR20	-2.77	<0.01
DKC1	-2.73	0.06
XIAP	-2.71	<0.01
RBM10	-2.71	<0.01
SPEN	-2.71	0.01
TRAF2	-2.68	0.01
RECQL5	-2.67	0.01
LEF1	-2.65	<0.01
MTA2	-2.62	<0.01
RHOH	-2.60	<0.01
ZBTB10	-2.60	0.02
SAMD4B	3.60	<0.01
RBM6	3.71	<0.01
CLK3	3.72	<0.01
HNRNPL	3.73	<0.01
IKZF1	3.83	<0.01
EIF4A1	3.83	<0.01
BTAF1	3.97	<0.01
G3BP1	4.03	<0.01
PTMA	4.17	<0.01
SPOP	4.64	<0.01
ZC3HAV1	4.77	<0.01
FLCN	4.87	<0.01
FNIP1	4.87	<0.01
CDK6	5.13	<0.01
TP53	5.29	<0.01
ZNF217	5.45	<0.01
CREB1	5.46	<0.01
CHD7	5.56	<0.01
ZBTB7A	5.92	<0.01
MTMR4	6.09	<0.01
IKZF2	6.20	<0.01
KHDRBS1	6.28	<0.01
ELAVL1	6.43	<0.01
CCNC	6.56	<0.01
CXorf38	7.25	<0.01
FBXO11	7.58	<0.01
TNFRSF10B	7.62	<0.01
FADD	8.49	<0.01
CASP8	8.49	<0.01
CASP3	8.50	<0.01

Screen	Correlation	Plot
TRAIL 5ng/ml R00 exp35	0.408	View
Thapsigargin 0.005μM R07 exp407	0.065	View
Tubacin 1.6μM R07 exp416	0.054	View
Wortmannin 5μM R00 exp38	0.053	View
3-Bromopyruvate 7μM R04 exp175	0.078	View
MLN-4924 0.2μM R00 exp21	0.073	View
Lead acetate 2000μM R08 exp497	0.058	View
Olaparib 4μM R08 exp512	0.058	View
Bortezomib 0.005μM R00 exp6	0.058	View
Aphidicolin 0.4μM R08 exp440	0.057	View
Lead acetate 2000μM R08 exp498 no dilution day6	0.055	View
Quercetin 20μM R08 exp517	0.055	View
AICAR 240μM R05 exp211	0.054	View
Hydroxyurea 100μM R07 exp329	0.052	View
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	0.052	View
I-BRD9 4μM R07 exp365	0.051	View

GO Term	Fold Change	Genes
GATOR1 complex	592.18	NPRL3,NPRL2
Seh1-associated complex	253.79	NPRL3,DEPDC5,NPRL2
negative regulation of TORC1 signaling	72.22	NPRL3,DEPDC5,SZT2,UBE2N,NPRL2
cellular response to amino acid starvation	50.40	NPRL3,DEPDC5,SZT2,NPRL2
response to amino acid starvation	47.37	NPRL3,DEPDC5,SZT2,NPRL2
negative regulation of TOR signaling	44.86	NPRL3,DEPDC5,SZT2,UBE2N,NPRL2
regulation of TORC1 signaling	34.43	NPRL3,DEPDC5,SZT2,UBE2N,NPRL2
regulation of TOR signaling	21.93	NPRL3,DEPDC5,SZT2,UBE2N,NPRL2
protein polyubiquitination	14.51	RNF19B,UBE2D3,UBE2N,XIAP,TRAF2
cellular response to nutrient levels	12.76	NPRL3,DEPDC5,SZT2,PRKAG1,NPRL2
positive regulation of cellular catabolic process	11.35	CNOT2,NPRL3,DEPDC5,CNOT8,NPRL2,RB1CC1
regulation of cellular catabolic process	8.28	CNOT2,NPRL3,DEPDC5,CNOT8,NPRL2,RB1CC1,DKC1,RBM10,TRAF2
positive regulation of catabolic process	8.06	CNOT2,NPRL3,DEPDC5,RNF19B,CNOT8,NPRL2,RB1CC1
DNA metabolic process	7.45	ZBTB1,ARID4B,UBE2D3,PDS5B,UBE2N,DKC1,RECQL5,LEF1,MTA2
kinase binding	6.20	BCL11A,GLRX3,MIDN,PRKAG1,RB1CC1,XIAP,TRAF2,RHOH
negative regulation of transcription by RNA polymerase II	6.14	CNOT2,ZBTB1,BCL11A,MBD3,ARID4B,UBE2D3,RBM10,SPEN,LEF1,MTA2

GO Term	Fold Change	Genes
death effector domain binding	368.47	CASP8,FADD
cysteine-type endopeptidase activity involved in apoptotic signaling pathway	276.35	CASP3,CASP8
glial cell apoptotic process	276.35	CASP3,TP53
CKM complex	276.35	CCNC,MED13
TRAIL-activated apoptotic signaling pathway	276.35	CASP8,FADD,TNFRSF10B
death-inducing signaling complex	207.26	CASP3,CASP8,FADD
death receptor binding	82.91	CASP3,CASP8,FADD
regulation of programmed necrotic cell death	55.27	CASP8,FADD,TP53
lymphocyte differentiation	13.62	FADD,ZBTB7A,CHD7,TP53,CDK6,FNIP1,IKZF1
mRNA binding	12.40	ELAVL1,KHDRBS1,TP53,G3BP1,EIF4A1,HNRNPL,SAMD4B
leukocyte differentiation	11.93	CASP8,FADD,ZBTB7A,CHD7,CREB1,TP53,CDK6,FNIP1,IKZF1
mononuclear cell differentiation	11.83	FADD,ZBTB7A,CHD7,TP53,CDK6,FNIP1,IKZF1
regulation of mRNA metabolic process	11.24	ELAVL1,KHDRBS1,ZBTB7A,ZC3HAV1,HNRNPL,SAMD4B
apoptotic signaling pathway	10.77	CASP3,CASP8,FADD,TNFRSF10B,TP53,FLCN
cellular response to external stimulus	10.17	CASP8,FADD,TNFRSF10B,TP53,FNIP1,FLCN
lymphocyte activation	9.55	CASP8,FADD,ZBTB7A,CHD7,TP53,CDK6,FNIP1,IKZF1
hemopoiesis	9.11	CASP3,CASP8,FADD,ZBTB7A,CHD7,CREB1,TP53,CDK6,FNIP1,FLCN,IKZF1
positive regulation of apoptotic process	7.94	CASP3,CASP8,FADD,TNFRSF10B,TP53,FNIP1,FLCN
leukocyte activation	7.58	CASP8,FADD,ZBTB7A,CHD7,TP53,CDK6,FNIP1,IKZF1