Genistein 15μM R07 exp360

Genistein 15μM R07 exp360

Mechanism of Action

Inhibits tyrosine kinases, phytoestrogen, toposiomerase II, pleiotropic effects

Class / Subclass 1: Signal Transduction / Kinase Inhibitor
Class / Subclass 2: Metabolism / Hormone Agonist
Class / Subclass 3: Gene Regulation / Nuclear Receptor Ligand

Technical Notes

Protein References

PubChem Name: Genistein
Synonyms: NPI 031L
CAS #: 446-72-0
PubChem CID: 5280961
IUPAC: 5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one
INCHI Name: InChI=1S/C15H10O5/c16-9-3-1-8(2-4-9)11-7-20-13-6-10(17)5-12(18)14(13)15(11)19/h1-7,16-18H
INCHI Key: TZBJGXHYKVUXJN-UHFFFAOYSA-N
Molecular Weight: 270.24
Canonical SMILES: C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O
Isomeric SMILES: N/A
Molecular Formula: C15H10O5

Protein Supplier

Supplier Name: Combi-Blocks
Catalog #: OR-2321
Lot #: L76364

Protein Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C15H10O5 271.0601; found 271.06011

Dose Response Curve

Platform ID: Genistein
Min: 0.3685; Max: 88.8447

IC	Concentration (µM)
IC10	N/A
IC20	13.3899
IC30	15.7760
IC40	18.1027
IC50	20.6354
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 07
Dose: 15µM
Days of incubation: 8
Doublings: 1.5
Numbers of reads: 20695605

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
14/51	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
ZNF451	-3.92	<0.01
TSC2	-3.31	<0.01
LIG4	-3.06	<0.01
MCM9	-3.04	<0.01
TCF4	-3.02	<0.01
PDS5B	-3.00	<0.01
TDP2	-2.98	<0.01
NHEJ1	-2.81	<0.01
H2AFX	-2.79	0.01
ASB3	-2.77	0.01
NPRL2	-2.76	<0.01
ATM	-2.69	0.01
TOP1	-2.68	0.01
PSMA2	-2.67	0.09
PSMA7	-2.64	0.09
PBRM1	-2.60	0.01
NPRL3	-2.53	0.09
MSI2	-2.51	0.06
PSMB7	-2.40	0.09
SWI5	-2.36	0.09
LEF1	-2.28	0.09
SMCHD1	-2.26	0.09
MCM8	-2.23	0.09
TP53BP1	-2.23	0.09
RAD9B	-2.23	0.09
CNOT2	-2.22	0.09
PUS7	-2.21	0.13
DCLRE1C	-2.21	0.09
UBE2N	-2.19	0.09
FAM35A	-2.17	0.09
NARS2	2.53	0.01
GLMN	2.53	0.01
PRKRIP1	2.54	0.01
KLF16	2.58	0.03
FKBP5	2.59	0.01
CCDC41	2.60	0.02
PPP1R8	2.62	0.02
DDX46	2.63	0.26
ZDHHC12	2.65	<0.01
CCNL1	2.67	<0.01
MTG1	2.67	0.01
NFU1	2.69	0.02
ATP1A1	2.69	0.01
NDUFAF3	2.78	0.02
NDUFB11	2.88	<0.01
GLRX5	2.89	<0.01
GPI	2.93	0.05
COPS7B	2.94	<0.01
WBP4	2.98	<0.01
ME2	3.00	<0.01
PIGO	3.07	<0.01
PQBP1	3.09	<0.01
NDUFB6	3.13	<0.01
UBXN7	3.24	<0.01
NDUFA11	3.29	<0.01
UBE2G1	3.54	<0.01
ERCC6	3.91	<0.01
SLC25A51	4.06	<0.01
TP53	4.32	<0.01
TOP2B	8.37	<0.01

Screen	Correlation	Plot
Hydroxyurea 100μM R07 exp329	0.103	View
HMS-I2 10μM R06 exp288	0.099	View
Pyridostatin 4μM R05 exp240	0.098	View
Oligomycin-A 2μM R00 exp25	0.088	View
UM0125461 0.74μM R02 exp84	0.084	View
Tubacin 1.6μM R07 exp416	0.078	View
Talazoparib 0.006μM R06 exp299	0.074	View
Thimerosal 0.85μM R08 exp529	0.069	View
Nutlin-3A 1.6μM R06 exp294	0.069	View
Pterostilbene 16μM R02 exp78	0.068	View
Quercetin 20μM R08 exp517	0.063	View
Ribavirin 10 to 15μM on day4 R04 exp160	0.055	View
Tozasertib 0.1μM R07 exp414	0.054	View
Rucaparib 6.5μM R08 exp520	0.053	View
Ixabepilone 0.004 to 0.005μM on day4 R04 exp184	0.052	View
Etoposide 0.1μM R05 exp198	0.111	View
Artemisinin 50μM R08 exp450	0.107	View
Calcium Ionophore 0.4μM R07 exp342	0.076	View
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	0.067	View
BML-284 0.09μM R08 exp460	0.063	View
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	0.062	View
BI-D1870 3.15μM R02 exp66	0.06	View
Atovaquone 15μM R08 exp451	0.058	View
Dorsomorphin 5μM R07 exp357	0.056	View
Doxorubicin 0.02μM R08 exp478	0.054	View
Aphidicolin 0.4μM R08 exp440	0.054	View
Deferoxamine 11μM R07 exp350	0.053	View
Combretastatin A4 0.002 to 0.003μM day4 R04 exp179	0.051	View
Epothilone-B 0.0015μM R05 exp231	0.051	View

GO Term	Fold Change	Genes
V(D)J recombination	168.14	LIG4,NHEJ1,ATM,LEF1,DCLRE1C
DNA repair complex	142.92	LIG4,NHEJ1,ATM,TP53BP1,DCLRE1C
somatic diversification of immune receptors via germline recombination within a single locus	69.72	LIG4,NHEJ1,ATM,LEF1,DCLRE1C
somatic cell DNA recombination	69.72	LIG4,NHEJ1,ATM,LEF1,DCLRE1C
double-strand break repair via nonhomologous end joining	68.06	LIG4,NHEJ1,ATM,TP53BP1,DCLRE1C
somatic diversification of immune receptors	58.34	LIG4,NHEJ1,ATM,LEF1,DCLRE1C
cellular response to ionizing radiation	51.20	LIG4,H2AX,ATM,SWI5,TP53BP1,RAD9B
response to ionizing radiation	34.39	LIG4,NHEJ1,H2AX,ATM,SWI5,TP53BP1,RAD9B,DCLRE1C
double-strand break repair	34.13	LIG4,MCM9,TDP2,NHEJ1,H2AX,ATM,SWI5,SMCHD1,MCM8,TP53BP1,DCLRE1C,UBE2N
regulation of TORC1 signaling	33.24	TSC2,NPRL2,ATM,NPRL3,UBE2N
double-strand break repair via homologous recombination	29.57	MCM9,H2AX,ATM,SWI5,MCM8,UBE2N
recombinational repair	28.82	MCM9,H2AX,ATM,SWI5,MCM8,UBE2N
DNA recombination	25.52	LIG4,MCM9,NHEJ1,H2AX,ATM,SWI5,LEF1,MCM8,DCLRE1C,UBE2N
cellular response to radiation	20.66	LIG4,H2AX,ATM,SWI5,TP53BP1,RAD9B
DNA repair	16.99	LIG4,MCM9,PDS5B,TDP2,NHEJ1,H2AX,ATM,SWI5,SMCHD1,MCM8,TP53BP1,RAD9B,DCLRE1C,UBE2N
DNA metabolic process	12.78	LIG4,MCM9,PDS5B,TDP2,NHEJ1,H2AX,ATM,TOP1,SWI5,LEF1,SMCHD1,MCM8,TP53BP1,RAD9B,DCLRE1C,UBE2N
DNA damage response	11.21	LIG4,MCM9,PDS5B,TDP2,NHEJ1,H2AX,ATM,SWI5,SMCHD1,MCM8,TP53BP1,RAD9B,DCLRE1C,UBE2N
response to radiation	11.15	LIG4,NHEJ1,H2AX,ATM,SWI5,TP53BP1,RAD9B,DCLRE1C
chromosome organization	10.39	LIG4,MCM9,PDS5B,ATM,TOP1,SMCHD1,MCM8,DCLRE1C

GO Term	Fold Change	Genes
mitochondrial respiratory chain complex I assembly	42.51	NDUFA11,NDUFB6,NDUFB11,NDUFAF3
NADH dehydrogenase complex assembly	42.51	NDUFA11,NDUFB6,NDUFB11,NDUFAF3
generation of precursor metabolites and energy	10.29	TP53,SLC25A51,NDUFA11,NDUFB6,ME2,GPI,NDUFB11

Table of Contents

Genistein 15μM R07 exp360

Mechanism of Action

Technical Notes

Protein References

Protein Supplier

Protein Characterization

Dose Response Curve

Screen Summary

Screen Results