TOP1MT
Gene Information
- Official Symbol: TOP1MT
- Official Name: DNA topoisomerase I mitochondrial
- Aliases and Previous Symbols: N/A
- Entrez ID: 116447
- UniProt: Q969P6
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: N/A
- UniProt Summary: Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). {ECO:0000250, ECO:0000269|PubMed:11526219}.
CRISPR Data
Essentiality in NALM6
- Essentiality Rank: 906
- Expression level (log2 read counts): 5.36
TOP1MT Expression in NALM6 Cells: 5.36
