LY2090314 0.003μM R08 exp500 no dilution day6

LY2090314 0.003μM R08 exp500 no dilution day6

Mechanism of Action

Inhibits glycogen synthase kinase-3 (GSK-3), activates Wnt signalling

Class / Subclass 1: Signal Transduction / Kinase Inhibitor

Technical Notes

Compound References

PubChem Name: 3-[6-Fluoro-10-(piperidine-1-carbonyl)-1,10-diazatricyclo[6.4.1.04,13]trideca-2,4,6,8(13)-tetraen-3-yl]-4-imidazo[1,2-a]pyridin-3-ylpyrrole-2,5-dione
Synonyms: N/A
CAS #: 603288-22-8
PubChem CID: 10029385
IUPAC: 3-[6-fluoro-10-(piperidine-1-carbonyl)-1,10-diazatricyclo[6.4.1.04,13]trideca-2,4,6,8(13)-tetraen-3-yl]-4-imidazo[1,2-a]pyridin-3-ylpyrrole-2,5-dione
INCHI Name: InChI=1S/C28H25FN6O3/c29-18-12-17-15-34(28(38)32-7-3-1-4-8-32)11-10-33-16-20(19(13-18)25(17)33)23-24(27(37)31-26(23)36)21-14-30-22-6-2-5-9-35(21)22/h2,5-6,9,12-14,16H,1,3-4,7-8,10-11,15H2,(H,31,36,37)
INCHI Key: HRJWTAWVFDCTGO-UHFFFAOYSA-N
Molecular Weight: 512.5
Canonical SMILES: C1CCN(CC1)C(=O)N2CCN3C=C(C4=CC(=CC(=C43)C2)F)C5=C(C(=O)NC5=O)C6=CN=C7N6C=CC=C7
Isomeric SMILES: N/A
Molecular Formula: C28H25FN6O3

Compound Supplier

Supplier Name: Med Chem Express
Catalog #: HY-16294
Lot #: 11009

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C28H25FN6O3 513.20449; found 513.208

Dose Response Curve

Platform ID: LY2090314
Min: -16.6945; Max: 87.6124

IC	Concentration (µM)
IC10	0.0026
IC20	0.0033
IC30	0.0039
IC40	0.0044
IC50	0.0050
IC60	0.0056
IC70	0.0064
IC80	0.0076
IC90	0.0096

Screen Summary

Round: 08
Dose: 3nM
Days of incubation: 8
Doublings: 3.6
Numbers of reads: 15503499

Screen Results

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
46/75	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
GSK3B	-5.52	0.00
NPRL3	-4.93	0.00
NPRL2	-4.14	0.00
SZT2	-4.05	0.00
RING1	-3.57	0.00
MSI2	-3.57	0.00
TCF4	-3.54	0.00
ITFG2	-3.47	0.00
C12orf66	-3.41	0.00
ZBTB24	-3.41	0.00
MAPRE1	-3.23	<0.01
PCBP2	-3.23	0.00
IVNS1ABP	-3.13	0.00
DEPDC5	-3.11	<0.01
PRKAA1	-3.00	<0.01
ILF3	-2.97	0.04
PRMT7	-2.97	<0.01
BACH2	-2.86	<0.01
CSTB	-2.86	<0.01
PRKCE	-2.84	<0.01
OTUB1	-2.84	<0.01
BCL6	-2.82	<0.01
INTS12	-2.72	<0.01
PRPS1	-2.70	<0.01
FLI1	-2.67	<0.01
AXIN1	-2.66	0.01
ELAVL1	-2.64	<0.01
UXS1	-2.62	<0.01
CCDC130	-2.60	<0.01
UBE4B	-2.56	<0.01
ALDH18A1	2.79	<0.01
HDAC1	2.79	<0.01
MAPKAP1	2.87	<0.01
PPP3CA	2.88	<0.01
TRIP12	2.89	<0.01
INO80C	2.89	<0.01
SLC12A2	2.91	<0.01
BAP1	2.91	<0.01
TIAL1	2.96	<0.01
DYRK1A	2.99	0.00
LDB1	3.11	0.00
PDS5A	3.27	0.00
ING1	3.38	0.00
RB1	3.42	0.00
FOXK1	3.70	0.00
SRM	3.72	0.00
NFRKB	3.85	0.00
TNKS2	4.14	0.00
ATP2B1	4.17	0.00
MLST8	4.32	0.00
ARID1A	4.33	0.00
NSD1	4.64	0.00
KLF16	4.64	0.00
RPTOR	4.67	0.00
DOHH	4.77	0.00
MBNL1	5.49	0.00
ID3	5.99	0.00
JUP	7.01	0.00
LEF1	7.36	0.00
CTNNB1	7.43	0.00

Screen	Correlation	Plot
MLN-4924 0.2μM R00 exp21	0.09	View
Quercetin 20μM R08 exp517	0.068	View
UM131593 0.2μM R03 exp106	0.067	View
Milciclib 2μM R08 exp502	0.064	View
UMK57 1μM R01 exp59	0.06	View
THZ1 0.06μM R08 exp531	0.057	View
Radicicol 0.16μM R05 exp242	0.057	View
Taxol 0.004μM R03 exp103	0.056	View
MS023 2μM R03 exp134	0.054	View
RO-3307 4.7μM R02 exp80	0.054	View
UM0125461 0.74μM R02 exp84	0.053	View
MS023 7μM R03 exp135	0.053	View
Sulforaphane 9μM R08 exp526 no dilution day6	0.053	View
ML-792 0.2μM R08 exp505	0.052	View
TRAIL 5ng/ml R00 exp35	0.052	View
Sulforaphane 9μM R08 exp525	0.052	View
Lead acetate 2000μM R08 exp497	0.052	View
LY2090314 0.003μM R08 exp499	0.448	View
3-Bromopyruvate 7μM R04 exp175	0.081	View
BVD-523 15μM R02 exp67	0.078	View
Aphidicolin 0.4μM R08 exp440	0.074	View
Hippuristanol 0.12μM R08 exp489 no dilution day6	0.068	View
JQ1 0.8μM R08 exp435	0.062	View
CB-5083 0.4μM R08 exp468	0.061	View
Ethanol 0.01 R05 exp234	0.061	View
Benzoate 20000μM R08 exp456	0.059	View
Artemisinin 50μM R08 exp450	0.059	View
AICAR 240μM R05 exp211	0.057	View
Combretastatin A4 0.002 to 0.003μM day4 R04 exp179	0.056	View
Epothilone-B 0.0015μM R05 exp231	0.056	View
5-Fluorouracil 20μM R00 exp2	0.055	View
Hippuristanol 0.12μM R08 exp488	0.055	View
Atovaquone 15μM R08 exp451	0.054	View
I-BRD9 4μM R07 exp365	0.053	View
BML-284 0.09μM R08 exp460	0.053	View
Bleomycin 5μM R08 exp459	0.052	View
Actinomycin-D 0.001μM R00 exp3	0.051	View

GO Term	Fold Change	Genes
GATOR1 complex	613.67	NPRL3,NPRL2
Seh1-associated complex	263.00	NPRL3,NPRL2,DEPDC5
negative regulation of TORC1 signaling	89.80	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1
cellular response to amino acid starvation	63.92	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5
response to amino acid starvation	60.16	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5
negative regulation of TOR signaling	55.79	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1
regulation of TORC1 signaling	49.95	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1,OTUB1
regulation of TOR signaling	27.27	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1
cellular response to starvation	21.53	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1
positive regulation of autophagy	21.02	GSK3B,NPRL3,NPRL2,DEPDC5,PRKAA1
response to starvation	17.53	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1
cellular response to nutrient levels	15.80	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1
cellular response to extracellular stimulus	14.05	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1
cellular response to external stimulus	11.26	NPRL3,NPRL2,SZT2,ITFG2,DEPDC5,PRKAA1
negative regulation of intracellular signal transduction	10.79	GSK3B,NPRL3,NPRL2,SZT2,ITFG2,IVNS1ABP,DEPDC5,PRKAA1,BCL6
positive regulation of catabolic process	8.34	GSK3B,NPRL3,NPRL2,DEPDC5,PRKAA1,PRKCE,AXIN1

GO Term	Fold Change	Genes
positive regulation of pentose-phosphate shunt	552.30	RPTOR,MLST8
beta-catenin-TCF complex	138.08	CTNNB1,LEF1,LDB1
tongue development	87.21	CTNNB1,LEF1,HDAC1
positive regulation of myeloid leukocyte differentiation	38.76	LEF1,RPTOR,RB1,PPP3CA
SWI/SNF superfamily-type complex	37.23	ARID1A,NFRKB,RB1,INO80C,GATAD2A,HDAC1
ATPase complex	23.84	ARID1A,NFRKB,RB1,INO80C,GATAD2A,HDAC1
regulation of myeloid leukocyte differentiation	23.40	CTNNB1,LEF1,RPTOR,RB1,PPP3CA
nuclear receptor binding	20.61	CTNNB1,LEF1,NSD1,ARID1A,TRIP12
regulation of myeloid cell differentiation	17.63	CTNNB1,LEF1,RPTOR,RB1,LDB1,PPP3CA
regulation of chromosome organization	13.69	CTNNB1,ARID1A,TNKS2,NFRKB,RB1,INO80C
RNA polymerase II-specific DNA-binding transcription factor binding	13.03	CTNNB1,LEF1,NSD1,ARID1A,RB1,LDB1,TRIP12,HDAC1
DNA-binding transcription factor binding	11.78	CTNNB1,LEF1,JUP,ID3,NSD1,ARID1A,RB1,LDB1,TRIP12,HDAC1
transcription factor binding	10.33	CTNNB1,LEF1,JUP,ID3,RPTOR,NSD1,ARID1A,RB1,LDB1,TRIP12,HDAC1
regulation of hemopoiesis	9.94	CTNNB1,LEF1,RPTOR,ARID1A,RB1,LDB1,PPP3CA
chromatin remodeling	9.81	NSD1,ARID1A,NFRKB,RB1,DYRK1A,BAP1,INO80C,TRIP12,GATAD2A,HDAC1
transcription coregulator activity	9.07	CTNNB1,JUP,NSD1,ARID1A,RB1,LDB1,DYRK1A,HDAC1
chromatin organization	7.83	NSD1,ARID1A,NFRKB,RB1,DYRK1A,BAP1,INO80C,TRIP12,GATAD2A,HDAC1
protein-DNA complex organization	7.05	NSD1,ARID1A,NFRKB,RB1,DYRK1A,BAP1,INO80C,TRIP12,GATAD2A,HDAC1
negative regulation of transcription by RNA polymerase II	6.32	CTNNB1,LEF1,ID3,KLF16,NSD1,FOXK1,RB1,ING1,LDB1,GATAD2A,HDAC1

Table of Contents

LY2090314 0.003μM R08 exp500 no dilution day6

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results