BUB3

Entrez Summary: This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
UniProt Summary: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. {ECO:0000269|PubMed:10198256, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:18199686}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
LY2090314 0.003μM R08 exp499	-2.26
Quercetin 20μM R08 exp517	-2.21
Fluvastatin 2.2μM R06 exp282	-2.12
2-Methoxyestradiol 0.55μM R07 exp310	-2.09
Vincristine 0.0005μM R04 exp190	-1.92
Ethambutol 25μM R08 exp481	-1.85
Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471	-1.84
All-trans-Retinoic-Acid 8μM R07 exp333	-1.81
Myriocin 25μM R06 exp293	-1.81
LY2090314 0.003μM R08 exp500 no dilution day6	-1.79
DKK1 89ng/ml R08 exp477	-1.72
Rapamycin 1μM R00 exp29	-1.71
Wortmannin 0.5μM R00 exp37	-1.71
Atovaquone 15μM R08 exp451	1.74
RO-3307 4.7μM R02 exp80	1.76
Tanespimycin 14μM R08 exp527	1.88
Etoposide 0.3μM R05 exp199	1.92
Vinblastine 0.2μM R03 exp108	3.04
GSK461364A 0.1μM R03 exp126	3.45
BI-2536 0.02μM R03 exp96	3.56
Cytochalasin-B 5μM R07 exp349	3.95
Staurosporine 0.02μM R08 exp524	4.04
Tozasertib 0.1μM R07 exp414	4.17
Centrinone 0.5μM R07 exp343	4.5
Latrunculin-B 10μM R07 exp374	4.81
DABN 20μM R00 exp17	4.85
HMS-I1 10μM R06 exp256	5.54
BI-6727 0.04μM R03 exp98	5.75
SB743921 0.001μM R05 exp244	5.75
Nocodazole 0.2μM R02 exp64	5.78
R-DABN 8μM R03 exp111	5.89
DABN racemic mixture R03 exp93	6
Nocodazole 0.1μM R03 exp94	6.01
Vinblastine 0.015μM R05 exp208	6.86
Nocodazole 0.2μM R00 exp24	6.9
Rotenone 2μM R00 exp33	6.9

Global Fraction of Cell Lines Where Essential: 735/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	31/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Expression Distribution