GEMIN5

Gene Information

Official Symbol: GEMIN5
Official Name: gem nuclear organelle associated protein 5
Aliases and Previous Symbols: N/A
Entrez ID: 25929
UniProt: Q8TEQ6
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
UniProt Summary: Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:16857593, PubMed:18984161, PubMed:20513430). Thereby, plays an important role in the splicing of cellular pre- mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161). Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (PubMed:18984161). GEMIN5 acts as the snRNA-binding protein of the SMN complex (PubMed:11714716, PubMed:16857593, PubMed:19377484, PubMed:19750007, PubMed:20513430, PubMed:27834343, PubMed:27881600, PubMed:27881601). Binds to the 7-methylguanosine cap of RNA molecules (PubMed:19750007, PubMed:27834343, PubMed:27881600, PubMed:27881601, Ref.25). Binds to the 3'-UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (PubMed:27507887). {ECO:0000269|PubMed:11714716, ECO:0000269|PubMed:16857593, ECO:0000269|PubMed:18984161, ECO:0000269|PubMed:19377484, ECO:0000269|PubMed:19750007, ECO:0000269|PubMed:20513430, ECO:0000269|PubMed:25911097, ECO:0000269|PubMed:27507887, ECO:0000269|PubMed:27834343, ECO:0000269|PubMed:27881600, ECO:0000269|PubMed:27881601, ECO:0000269|Ref.25}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Nocodazole 0.02μM R00 exp23	-2.03
Pimelic-diphenylamide-106 0.5μM R00 exp27	-2.02
FK-506 30μM R07 exp359	-1.98
UM0011500 5μM R05 exp245	-1.95
HMS-I2 5μM R06 exp287	-1.93
QNZ 0.01μM R05 exp241	-1.85
Etoposide 0.1μM R05 exp198	-1.84
Radicicol 0.16μM R05 exp242	-1.82
Oligomycin-A 2μM R00 exp25	-1.81
NMS-873 0.07μM R07 exp380	-1.79
Epigallocatechin gallate 20μM R05 exp230	-1.77
5-Fluorouracil 2μM R00 exp1	-1.76
BS-181 20μM R08 exp461	-1.76
Chloramphenicol 20μM R00 exp13	-1.71

Gene	Correlation
HARS	0.696
GTF3C2	0.55
PMM2	0.53
NSMCE1	0.529
DDX20	0.525
GMPPB	0.525
NAE1	0.516
RTCB	0.51
DPAGT1	0.509
RPP21	0.504
YARS	0.502
PSMG4	0.498
GTF3C3	0.488
RNMT	0.481
ALG14	0.481
ENO1	0.48
GFPT1	0.476
VEZT	0.475
UTP23	0.475
TRIAP1	0.472
RAD1	0.469
TGS1	0.464
THG1L	0.459
PELO	0.457
CCT7	0.456
EXOC3	0.453
TRMT5	0.453
UAP1	0.452
ALG2	0.452
GTF3C6	0.45
VRK1	0.45
UXT	0.449
NARS	0.449
EPRS	0.448
SNAPC5	0.447
ALG1	0.447
SRP14	0.447
TXNL4B	0.445
RPE	0.444
SRP68	0.443
ATXN10	0.435
TELO2	0.433
DAD1	0.432
UHRF1	0.431
HAUS8	0.429
GEMIN8	0.429
PAM16	0.428
ADSL	0.428
RABGGTB	0.426
OSGEP	0.426
GTF3C4	0.426
ZRSR2	0.425
MARS	0.423
FNTA	0.422
TTI2	0.421
MED16	0.418
MAD2L1	0.418
ALG13	0.418
DDX55	0.417
RBM14	0.416
TSEN54	0.411
TBCA	0.41
AARS	0.407
WDR82	0.406
ZNF259	0.405
SDHA	0.405
EXOC4	0.403
OIP5	0.401
ENY2	0.401
NHP2	0.401
GPN2	0.401

Global Fraction of Cell Lines Where Essential: 732/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	25/26
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	20/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	14/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 1345
Expression level (log2 read counts): 6.93

Expression Distribution

SMN-Gemin2 complex
U4atac snRNA binding
U4 snRNA binding
snRNA binding
U1 snRNA binding
SMN complex
Gemini of coiled bodies
RNA 7-methylguanosine cap binding
SMN-Sm protein complex
spliceosomal snRNP assembly
ribosome binding
cytosolic large ribosomal subunit
mRNA 3-UTR binding
import into nucleus
ribonucleoprotein complex assembly
ribonucleoprotein complex subunit organization
nucleocytoplasmic transport
nuclear transport
nuclear body
mRNA splicing, via spliceosome
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
RNA splicing, via transesterification reactions
regulation of translation
translation
RNA splicing
regulation of cellular amide metabolic process
peptide biosynthetic process
ribonucleoprotein complex biogenesis
mRNA processing
posttranscriptional regulation of gene expression
amide biosynthetic process
peptide metabolic process
mRNA metabolic process
cellular amide metabolic process
cellular protein-containing complex assembly
RNA processing
RNA binding
organonitrogen compound biosynthetic process
intracellular transport
protein-containing complex assembly