BUB3

Gene Information

Official Symbol: BUB3
Official Name: BUB3 mitotic checkpoint protein
Aliases and Previous Symbols: N/A
Entrez ID: 9184
UniProt: O43684
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
UniProt Summary: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. {ECO:0000269|PubMed:10198256, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:18199686}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
LY2090314 0.003μM R08 exp499	-2.26
Quercetin 20μM R08 exp517	-2.21
Fluvastatin 2.2μM R06 exp282	-2.12
2-Methoxyestradiol 0.55μM R07 exp310	-2.09
Vincristine 0.0005μM R04 exp190	-1.92
Ethambutol 25μM R08 exp481	-1.85
Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471	-1.84
All-trans-Retinoic-Acid 8μM R07 exp333	-1.81
Myriocin 25μM R06 exp293	-1.81
LY2090314 0.003μM R08 exp500 no dilution day6	-1.79
DKK1 89ng/ml R08 exp477	-1.72
Rapamycin 1μM R00 exp29	-1.71
Wortmannin 0.5μM R00 exp37	-1.71
Atovaquone 15μM R08 exp451	1.74
RO-3307 4.7μM R02 exp80	1.76
Tanespimycin 14μM R08 exp527	1.88
Etoposide 0.3μM R05 exp199	1.92
Vinblastine 0.2μM R03 exp108	3.04
GSK461364A 0.1μM R03 exp126	3.45
BI-2536 0.02μM R03 exp96	3.56
Cytochalasin-B 5μM R07 exp349	3.95
Staurosporine 0.02μM R08 exp524	4.04
Tozasertib 0.1μM R07 exp414	4.17
Centrinone 0.5μM R07 exp343	4.5
Latrunculin-B 10μM R07 exp374	4.81
DABN 20μM R00 exp17	4.85
HMS-I1 10μM R06 exp256	5.54
BI-6727 0.04μM R03 exp98	5.75
SB743921 0.001μM R05 exp244	5.75
Nocodazole 0.2μM R02 exp64	5.78
R-DABN 8μM R03 exp111	5.89
DABN racemic mixture R03 exp93	6
Nocodazole 0.1μM R03 exp94	6.01
Vinblastine 0.015μM R05 exp208	6.86
Nocodazole 0.2μM R00 exp24	6.9
Rotenone 2μM R00 exp33	6.9

Gene	Correlation
MAD1L1	0.801
MAD2L1	0.799
RBM14	0.763
KNTC1	0.72
ZW10	0.707
ZWILCH	0.684
TRIP13	0.678
BUB1B	0.663
MAD2L1BP	0.621
MED16	0.594
FNTA	0.591
PELO	0.579
FNTB	0.56
C14orf80	0.541
DBR1	0.517
RNMT	0.509
DPAGT1	0.508
THG1L	0.502
TUBD1	0.5
DDX20	0.485
C16orf59	0.476
ALG1	0.471
GNB1L	0.464
RTCB	0.464
SRP72	0.45
TIMM22	0.448
TTI2	0.447
SRP14	0.445
SNAPC5	0.444
PPP4C	0.443
TSEN54	0.442
GTF3C4	0.441
ORAOV1	0.44
UAP1	0.436
CDIPT	0.43
TUBE1	0.429
TGS1	0.427
GEMIN8	0.426
HSPD1	0.426
TMEM41B	0.425
ATXN10	0.421
ADSL	0.42
RTTN	0.418
OSGEP	0.417
TTI1	0.415
GTF3C2	0.415
NSMCE1	0.414
PAM16	0.413
WDR1	0.413
POLR3H	0.412
GEMIN6	0.411
RPP21	0.411
PMM2	0.41
DCTN6	0.409
TBCA	0.406
RABGGTB	0.403
CIAO1	0.403
DHPS	0.402
CCDC115	0.401
TRMT5	0.4

Global Fraction of Cell Lines Where Essential: 735/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	31/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 148
Expression level (log2 read counts): 8.01

Expression Distribution

bub1-bub3 complex
mitotic checkpoint complex
protein localization to kinetochore
protein localization to chromosome, centromeric region
attachment of spindle microtubules to kinetochore
mitotic spindle assembly checkpoint
spindle checkpoint
spindle assembly checkpoint
mitotic spindle checkpoint
negative regulation of mitotic metaphase/anaphase transition
negative regulation of metaphase/anaphase transition of cell cycle
negative regulation of mitotic sister chromatid separation
negative regulation of chromosome separation
negative regulation of mitotic sister chromatid segregation
negative regulation of sister chromatid segregation
negative regulation of chromosome segregation
negative regulation of mitotic nuclear division
negative regulation of nuclear division
regulation of mitotic metaphase/anaphase transition
regulation of metaphase/anaphase transition of cell cycle
regulation of mitotic sister chromatid separation
protein localization to chromosome
regulation of chromosome separation
regulation of mitotic sister chromatid segregation
ubiquitin binding
regulation of sister chromatid segregation
anaphase-promoting complex-dependent catabolic process
kinetochore
condensed chromosome kinetochore
regulation of chromosome segregation
mitotic sister chromatid segregation
negative regulation of chromosome organization
sister chromatid segregation
mitotic nuclear division
mitotic cell cycle checkpoint
regulation of mitotic nuclear division
regulation of nuclear division
cell cycle checkpoint
negative regulation of mitotic cell cycle phase transition
nuclear chromosome segregation