SRD5A3

Entrez Summary: The protein encoded by this gene belongs to the steroid 5-alpha reductase family, and polyprenol reductase subfamily. It is involved in the production of androgen 5-alpha-dihydrotestosterone (DHT) from testosterone, and maintenance of the androgen-androgen receptor activation pathway. This protein is also necessary for the conversion of polyprenol into dolichol, which is required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-linked glycosylation of proteins. Mutations in this gene are associated with congenital disorder of glycosylation type Iq. [provided by RefSeq, Mar 2011].
UniProt Summary: Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT). {ECO:0000269|PubMed:17986282, ECO:0000269|PubMed:20637498}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Bisphenol F 50μM R08 exp457	-1.99
Cannabidiol 13μM R08 exp465	-1.82
Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307	1.7
Atovaquone 15μM R08 exp451	1.71
ONC212 0.15μM R08 exp513	1.73
Pterostilbene 16μM R02 exp78	1.73
2-Deoxy-D-glucose 800μM R05 exp214	1.85
42°C R08 exp539	1.89
Wortmannin 5μM R00 exp38	1.91
Mubritinib 0.2μM R02 exp65	1.94
CFI-400945 25μM R08 exp469	1.96
Monensin 0.3μM R08 exp507	1.96
Milciclib 2μM R08 exp502	2.02
PF-06409577 20μM R07 exp389	2.08
GSK-J4 1-1.25μM to day4 R07 exp363	2.12
TIC10 10μM R08 exp532	2.21
WYE-354 6μM R02 exp90	2.21
Senexin-A 25μM R07 exp400	2.29
R-DABN 8μM R03 exp111	2.57

Global Fraction of Cell Lines Where Essential: 11/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	1/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	1/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	1/20
lung	1/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	1/16
plasma cell	0/15
prostate	0/1
skin	1/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

polyprenol reductase activity
dolichol biosynthetic process
polyprenol biosynthetic process
3-oxo-5-alpha-steroid 4-dehydrogenase activity
polyprenol catabolic process
oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
cholestenone 5-alpha-reductase activity
dolichol metabolic process
dolichyl diphosphate biosynthetic process
dolichyl diphosphate metabolic process
polyprenol metabolic process
isoprenoid catabolic process
androgen biosynthetic process
dolichol-linked oligosaccharide biosynthetic process
oligosaccharide-lipid intermediate biosynthetic process
androgen metabolic process
isoprenoid biosynthetic process
steroid hormone biosynthetic process
alcohol catabolic process
hormone biosynthetic process
organic hydroxy compound catabolic process
protein N-linked glycosylation
alcohol biosynthetic process
cellular hormone metabolic process
steroid biosynthetic process
isoprenoid metabolic process
organic hydroxy compound biosynthetic process
cellular lipid catabolic process
hormone metabolic process
phospholipid biosynthetic process
protein glycosylation
macromolecule glycosylation
steroid metabolic process
glycosylation
lipid catabolic process
alcohol metabolic process
glycoprotein biosynthetic process
phospholipid metabolic process
glycoprotein metabolic process
small molecule catabolic process