ENO1

Gene Information

Official Symbol: ENO1
Official Name: enolase 1
Aliases and Previous Symbols: N/A
Entrez ID: 2023
UniProt: P06733
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011].
UniProt Summary: N/A

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
∆-9-Tetrahydrocannabinol 30μM R08 exp445	-3.45
Phenformin 20μM R05 exp212	-2.91
THZ531 0.225μM R08 exp444	-2.82
Parthenolide 2.5μM R05 exp238	-2.6
THZ531 0.11μM R07 exp409	-2.5
SGC0649 7μM R03 exp150	-2.44
A-196 10μM R03 exp114	-2.41
THZ531 0.11 to 0.125μM on day4 R07 exp410	-2.41
Phenformin 20μM R03 exp143	-2.4
Calcium Ionophore 0.4μM R07 exp342	-2.32
NMS-873 0.07μM R07 exp380	-2.27
BAY-598 4μM R05 exp220	-2.18
MKC9989 10μM R03 exp133	-2.09
ABT-702 5μM plus CoCl2 18μM R07 exp320	-2.05
Metformin 100 to 150μM on day4 R04 exp170	-2.04
QNZ 0.01μM R05 exp241	-2.04
NFN1 0.4μM R03 exp99	-2.03
Hypoxia 5%O2 R04 exp191	-2
Doxycycline 10μM R00 exp18	-1.97
CoCl2 18μM R07 exp346	-1.95
Dihydrosphingosine 8μM R08 exp476	-1.93
Apcin 50 to 100μM on day4 R04 exp176	-1.92
CCCP 1μM R00 exp11	-1.92
PT-1 25μM R07 exp392	-1.91
Taxol 0.004μM R03 exp103	-1.89
THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412	-1.88
Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53	-1.85
Beta-Estradiol 0.55μM R07 exp425	-1.84
Geldanamycin 0.01μM R05 exp235	-1.84
Oligomycin-A 2μM R00 exp25	-1.84
Trichostatin-A 0.06μM R07 exp415	-1.84
Combretastatin A4 0.002 to 0.003μM day4 R04 exp179	-1.83
Geldanamycin 0.015 to 0.025μM on day4 R07 exp316	-1.8
NFN1 1μM R03 exp100	-1.78
Cabazitaxel 0.001μM R05 exp223	-1.77
A-395N 10μM R05 exp219	-1.76
Ribavirin 10 to 15μM on day4 R04 exp160	-1.76
Nicotinate 1000μM R03 exp140	-1.74
Isoniazid 100μM R08 exp494	-1.73
Pimelic-diphenylamide-106 0.5μM R00 exp27	-1.73
Asunaprenir 3μM R07 exp336	-1.72
Trametinib 10μM R02 exp83	-1.72
Rapamycin 2μM R07 exp306	1.71
2-Deoxy-D-glucose 800μM R05 exp214	1.72
DABN 20μM R00 exp17	1.79
MLN-4924 2μM R00 exp22	1.85
LJH685 50μM R08 exp433	1.99
Salinomycin 3.5μM R08 exp521	2.23
Cytochalasin-B 5μM R07 exp349	2.3
CFI-400945 25μM R08 exp469	2.34
Actinomycin-D 0.01μM R00 exp4	2.83
Bortezomib 0.05μM R00 exp7	3.35

Gene	Correlation
RPE	0.57
HARS	0.54
TPI1	0.533
PGK1	0.528
PKM	0.528
TRIAP1	0.518
ALG1	0.517
PMM2	0.515
THG1L	0.514
SRP14	0.509
NSMCE1	0.496
DPAGT1	0.494
DDX20	0.488
SLC25A26	0.486
GEMIN5	0.48
GMPPB	0.479
CRLS1	0.479
TKT	0.479
AASDHPPT	0.478
TBCA	0.474
RAD1	0.47
UHRF1	0.47
RTCB	0.47
GFPT1	0.465
GEMIN8	0.465
MARS	0.463
RNMT	0.454
COPS8	0.45
RPTOR	0.447
ADSL	0.445
UAP1	0.444
TGS1	0.441
MEAF6	0.44
DOHH	0.44
PPP2R3C	0.438
DDX55	0.437
CMTR1	0.435
NAE1	0.434
CSNK2B	0.432
CCT7	0.431
GTF3C6	0.429
GTF3C3	0.426
GTF3C2	0.425
RPIA	0.425
THOC3	0.422
NAA20	0.421
ROMO1	0.42
AARS	0.419
HSPD1	0.418
RBMX2	0.418
TMEM41B	0.417
PLAA	0.416
UTP23	0.416
TBP	0.415
CMTR2	0.414
WDR92	0.413
EXOC3	0.412
TAMM41	0.411
EIF4E	0.411
EXOC4	0.41
ZNHIT1	0.409
TEN1	0.409
VEZT	0.409
N6AMT1	0.407
TAZ	0.405
RABGGTB	0.403
ORAOV1	0.402
RAD50	0.402
PIK3R4	0.4

Global Fraction of Cell Lines Where Essential: 152/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	7/28
blood	4/28
bone	4/25
breast	6/33
central nervous system	9/56
cervix	2/4
colorectal	4/17
esophagus	4/13
fibroblast	0/1
gastric	3/15
kidney	4/21
liver	4/20
lung	18/75
lymphocyte	3/14
ovary	4/26
pancreas	4/24
peripheral nervous system	2/16
plasma cell	2/15
prostate	0/1
skin	6/24
soft tissue	1/7
thyroid	1/2
upper aerodigestive	13/22
urinary tract	9/29
uterus	1/5

Essentiality in NALM6

Essentiality Rank: 5
Expression level (log2 read counts): 9.92

Expression Distribution

Enolase C
Enolase N

phosphopyruvate hydratase complex
phosphopyruvate hydratase activity
regulation of hypoxia-induced intrinsic apoptotic signaling pathway
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
positive regulation of plasminogen activation
negative regulation of cellular response to hypoxia
positive regulation of ATP biosynthetic process
cell cortex region
regulation of cellular response to hypoxia
regulation of plasminogen activation
regulation of ATP biosynthetic process
positive regulation of purine nucleotide biosynthetic process
positive regulation of nucleotide biosynthetic process
positive regulation of protein processing
positive regulation of protein maturation
glucose catabolic process to pyruvate
NADH regeneration
canonical glycolysis
M band
glycolytic process through fructose-6-phosphate
glycolytic process through glucose-6-phosphate
glucose catabolic process
GTPase binding
NADH metabolic process
regulation of purine nucleotide biosynthetic process
regulation of nucleotide biosynthetic process
glycolytic process
ATP generation from ADP
gluconeogenesis
positive regulation of purine nucleotide metabolic process
positive regulation of muscle contraction
positive regulation of ATP metabolic process
positive regulation of nucleotide metabolic process
NAD metabolic process
hexose biosynthetic process
ADP metabolic process
hexose catabolic process
monosaccharide biosynthetic process
nucleoside diphosphate phosphorylation
nucleotide phosphorylation