TYMS

Gene Information

Official Symbol: TYMS
Official Name: thymidylate synthetase
Aliases and Previous Symbols: N/A
Entrez ID: 7298
UniProt: P04818
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017].
UniProt Summary: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. {ECO:0000269|PubMed:21876188}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Vemurafenib 6.6μM R08 exp434	-2.38
DABN 20μM R00 exp17	-2.01
Brefeldin A 0.02μM R00 exp8	-1.92
Rotenone 2μM R00 exp33	-1.9
Vinblastine 0.015μM R05 exp208	-1.84
Vinblastine 0.2μM R03 exp108	-1.74
Adefovir 20μM R07 exp332	1.71
BS-181 20μM R08 exp461	1.75
TGF-beta1 44ng/ml R08 exp528	1.75
Calcium Ionophore 0.4μM R07 exp342	1.76
3-Bromopyruvate 7μM R04 exp175	1.97
Thioridazine 5μM R08 exp530	2.1
GSK-J5 1.5μM R08 exp484	2.62

Gene	Correlation
POLR2J3	0.712
PRIM1	0.571
AATF	0.551
TCEB3CL	0.548
TCEB3C	0.541
TCEB3CL2	0.541
PSMB2	0.538
PSMB4	0.527
DTL	0.518
AQR	0.516
ATP6V1B2	0.515
MED14	0.513
VPS28	0.512
TICRR	0.51
CCNA2	0.502
SLC4A1	0.501
NPIPB5	0.497
COPG1	0.496
TBC1D3H	0.495
CBWD3	0.495
GOLGA6L1	0.493
CBWD6	0.491
NUPL1	0.487
EXOSC6	0.484
GAR1	0.484
TBC1D3C	0.482
RRP7A	0.482
COL2A1	0.481
MED17	0.48
TBC1D3I	0.479
TBC1D3E	0.478
C14orf93	0.478
ATP6V1A	0.478
TBC1D3L	0.477
TBC1D3K	0.477
TBC1D3F	0.477
TBC1D3G	0.476
COPB1	0.476
NSF	0.475
BYSL	0.474
TFIP11	0.472
IK	0.471
CBWD5	0.466
RPS2	0.466
SF3A1	0.466
POLR1A	0.462
CHAF1A	0.462
TBC1D3	0.461
MT1E	0.461
PSMD3	0.459
CHD4	0.458
POLE	0.458
CACTIN	0.457
C1QTNF9B	0.456
DOCK6	0.455
ATP6AP1	0.455
AKT1S1	0.455
DHX38	0.455
UTP3	0.454
DDX47	0.451
NOL12	0.451
ANKLE2	0.449
TIMELESS	0.449
EXOSC9	0.447
TWISTNB	0.447
ORC1	0.445
WDR75	0.443
PSMB1	0.442
CDCA3	0.441
POLD1	0.44
PSMC6	0.438
PSMC2	0.438
MDN1	0.437
PSMA6	0.437
SNIP1	0.434
CEP192	0.434
SUPT5H	0.433
PSMD7	0.433
UTP20	0.431
SMTN	0.431
CHCHD3	0.431
IGFN1	0.43
ANKLE1	0.43
DNTTIP2	0.43
ORC6	0.43
EXOSC3	0.43
PAN3	0.428
XAB2	0.428
HIST2H3C	0.428
HIST2H3A	0.428
UPF1	0.428
RPLP1	0.427
PLXNB3	0.427
RRP15	0.426
CDH24	0.425
ATP6AP2	0.425
TRAPPC8	0.423
RPL35	0.419
NOL10	0.419
ITGAX	0.419
GID4	0.418
RPS12	0.417
SMG1	0.416
POLR2D	0.416
USP35	0.416
POLR2J	0.416
OGFR	0.416
ATP6V1C1	0.415
NUP153	0.415
PHF2	0.415
NUP160	0.415
SLC6A17	0.411
TAF1	0.41
TP53TG3C	0.41
TP53TG3	0.41
TP53TG3B	0.41
PLEKHA4	0.409
POLR2F	0.409
NARFL	0.408
RFXANK	0.407
RPL6	0.406
ZNF830	0.406
LRR1	0.406
VASN	0.405
SNRPA1	0.405
ATP6V0C	0.405
PSMD2	0.405
GBF1	0.404
HIVEP3	0.404
COX7A2L	0.404
HEATR1	0.403
OSBP	0.403
COPA	0.403
GOLGA6L4	0.403
RPA2	0.402
DISP2	0.402
FAM231C	0.401
KCTD17	0.401
FAM231A	0.4

Global Fraction of Cell Lines Where Essential: 166/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	8/28
blood	14/28
bone	5/26
breast	15/33
central nervous system	6/56
cervix	1/4
colorectal	3/17
esophagus	2/13
fibroblast	0/1
gastric	5/16
kidney	3/21
liver	3/20
lung	21/75
lymphocyte	0/16
ovary	2/26
pancreas	6/24
peripheral nervous system	5/16
plasma cell	4/15
prostate	1/1
skin	5/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	4/22
urinary tract	4/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 59
Expression level (log2 read counts): 8.07

Expression Distribution

immortalization of host cell by virus
immortalization of host cell
thymidylate synthase activity
uracil metabolic process
transformation of host cell by virus
dTMP metabolic process
dTMP biosynthetic process
dTTP biosynthetic process
intestinal epithelial cell maturation
pyrimidine deoxyribonucleoside triphosphate biosynthetic process
dTTP metabolic process
deoxyribonucleoside triphosphate biosynthetic process
dUMP metabolic process
pyrimidine deoxyribonucleoside monophosphate biosynthetic process
pyrimidine deoxyribonucleoside monophosphate metabolic process
pyrimidine deoxyribonucleoside triphosphate metabolic process
columnar/cuboidal epithelial cell maturation
pyrimidine deoxyribonucleotide biosynthetic process
response to folic acid
deoxyribonucleoside monophosphate biosynthetic process
intestinal epithelial cell development
tetrahydrofolate interconversion
sequence-specific mRNA binding
deoxyribose phosphate biosynthetic process
folic acid binding
2-deoxyribonucleotide biosynthetic process
deoxyribonucleoside monophosphate metabolic process
deoxyribonucleotide biosynthetic process
translation repressor activity, mRNA regulatory element binding
pyrimidine nucleoside monophosphate biosynthetic process
epithelial cell maturation
pyrimidine nucleoside monophosphate metabolic process
deoxyribonucleoside triphosphate metabolic process
cofactor binding
tetrahydrofolate metabolic process
pyrimidine nucleoside triphosphate biosynthetic process
response to vitamin A
intestinal epithelial cell differentiation
pyrimidine deoxyribonucleotide metabolic process
pyrimidine nucleobase metabolic process