DNAJC3

Gene Information

Official Symbol: DNAJC3
Official Name: DnaJ heat shock protein family (Hsp40) member C3
Aliases and Previous Symbols: N/A
Entrez ID: 5611
UniProt: Q13217
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a protein with multiple tetratricopeptide repeat (TPR) motifs as well as the highly conserved J domain found in DNAJ chaperone family members. It is a member of the tetratricopeptide repeat family of proteins and acts as an inhibitor of the interferon-induced, dsRNA-activated protein kinase (PKR). [provided by RefSeq, Jul 2010].
UniProt Summary: Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. {ECO:0000250|UniProtKB:Q27968, ECO:0000250|UniProtKB:Q91YW3, ECO:0000269|PubMed:12601012, ECO:0000269|PubMed:8576172, ECO:0000269|PubMed:9447982, ECO:0000269|PubMed:9920933}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307	-3.72
Tunicamycin 0.04 to 0.125μM on day4 R07 exp420	-3.7
Golgicide-A 4μM R03 exp122	-3.64
Cyclosporin-A 3μM R07 exp348	-3.42
Tunicamycin 0.04μM R07 exp418	-3.37
Dynasore 10μM R04 exp180	-3.29
Dynasore 7μM R08 exp436	-3.26
Thapsigargin 0.005μM R07 exp407	-3.07
LB-100 2μM R05 exp210	-2.86
Trametinib 10μM R02 exp83	-2.81
Ethanol 0.01 R05 exp234	-2.79
B02 10μM R08 exp453	-2.59
A-395 10μM R05 exp218	-2.55
Cyclosporin-A 0.8μM R07 exp347	-2.4
ZLN024 50μM R08 exp538	-2.4
Cryptotanshinone 12μM R05 exp227	-2.39
Tunicamycin 0.04 to 0.075μM on day4 R07 exp419	-2.29
Lapatinib 5μM R01 exp47	-2.24
Benzoate 10000μM R08 exp455	-2.16
Bisphenol F 50μM R08 exp457	-2.15
All-trans-Retinoic-Acid 8μM R07 exp333	-2.05
Dimethyl-Sulfoxide 1pc R03 exp120	-2.03
ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319	-2.01
Rapamycin 10μM R00 exp30	-2.01
UM0011500 5μM R05 exp245	-2.01
Rifampicin 30μM R08 exp430	-1.97
Bortezomib 0.005μM R00 exp6	-1.96
SGC0946 7μM R03 exp151	-1.94
Aphidicolin 0.04μM R06 exp263	-1.9
Celastrol 0.12μM R05 exp225	-1.88
YM155 0.0002μM R01 exp61	-1.8
NFN1 0.1μM R01 exp49	-1.78
Dimethyloxaloylglycine 100μM R05 exp229	-1.77
proTAME 5μM R04 exp187	-1.77
2-Methoxyestradiol 0.2μM R01 exp40	-1.76
Lead acetate 2000μM R08 exp498 no dilution day6	-1.75
Caffeine 2600μM R08 exp463	-1.72
CFI-400945 25μM R08 exp469	1.8
Vinblastine 0.2μM R03 exp108	1.82
5-Fluorouracil 20μM R00 exp2	1.83
Nocodazole 0.02μM R00 exp23	1.83
BI-6727 0.04μM R03 exp98	1.85
UM131593 0.2μM R03 exp106	1.85
Actinomycin-D 0.01μM R00 exp4	1.87
Brefeldin A 0.02μM R00 exp8	1.96
SNS-032 15μM R08 exp443	2.08
Etoposide 0.3μM R05 exp199	2.25
GSK-J4 1-1.25μM to day4 R07 exp363	2.32
Etoposide 1μM R00 exp19	2.37
Q15 2.7μM R02 exp79	2.62
Atovaquone 15μM R08 exp451	2.67

Gene	Correlation
MANF	0.73
UBE2J1	0.665
DNAJB11	0.598
ALG5	0.593
DERL1	0.579
UBE2G2	0.578
DERL2	0.563
TMED10	0.556
IRF2BP2	0.528
ALG6	0.517
SEC62	0.507
TMEM41B	0.505
ENY2	0.502
UFM1	0.502
VMP1	0.502
XPO6	0.502
RNMT	0.5
SLC35B1	0.496
DPAGT1	0.494
ALG9	0.493
C1orf27	0.487
THOC6	0.485
UBA5	0.481
CBFB	0.48
DDX5	0.478
MBTPS2	0.478
ALG1	0.476
MLLT1	0.475
PMM2	0.475
CCDC101	0.475
ATP5O	0.474
SEC63	0.471
GTF3C2	0.462
TRMT10A	0.46
OS9	0.46
SLC33A1	0.459
BAP1	0.452
NXT1	0.452
KCMF1	0.452
MAPK14	0.451
GMPPB	0.451
RPTOR	0.45
ATP5J	0.449
DPM3	0.448
PPP2R3C	0.448
ALG3	0.445
TTI1	0.445
CSNK2B	0.44
MED16	0.439
GTF3C4	0.438
GFPT1	0.438
NUPL2	0.437
FLI1	0.436
UFC1	0.435
OTUD5	0.435
UAP1	0.431
SRP68	0.428
DHX29	0.427
AUP1	0.423
OPA1	0.42
ATP5A1	0.419
PDAP1	0.418
ATP5B	0.417
XPOT	0.416
EIF1	0.416
VCPIP1	0.416
LSMD1	0.415
ALG8	0.415
MED25	0.414
ATP5D	0.414
DHRSX	0.413
SOX7	0.413
TRIAP1	0.412
HSP90B1	0.411
HSPD1	0.411
HARS	0.408
MEAF6	0.408
ALG2	0.408
POLR3C	0.406
DAD1	0.404
TLE4	0.404
DOHH	0.404
ATP2A2	0.404
FAF2	0.404
SEC16A	0.403
DHDDS	0.402
DOLK	0.402
ALG12	0.402
AARS	0.402
ID3	0.401
WDR24	0.401
RPS6KB1	0.401
SMG9	0.4

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 4356
Expression level (log2 read counts): 6.2

Expression Distribution

TPR 1
DnaJ

positive regulation of translation initiation in response to endoplasmic reticulum stress
positive regulation of translation in response to endoplasmic reticulum stress
regulation of translation initiation in response to endoplasmic reticulum stress
negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation
positive regulation of translational initiation in response to stress
positive regulation of translation in response to stress
regulation of translation in response to endoplasmic reticulum stress
cellular response to cold
regulation of translational initiation in response to stress
regulation of translation in response to stress
protein folding in endoplasmic reticulum
misfolded protein binding
smooth endoplasmic reticulum
protein kinase inhibitor activity
positive regulation of translational initiation
negative regulation of response to endoplasmic reticulum stress
response to cold
extracellular vesicle
IRE1-mediated unfolded protein response
regulation of translational initiation
regulation of response to endoplasmic reticulum stress
azurophil granule lumen
chaperone binding
endoplasmic reticulum unfolded protein response
positive regulation of translation
cellular response to unfolded protein
unfolded protein binding
cellular response to topologically incorrect protein
positive regulation of cellular amide metabolic process
response to unfolded protein
response to temperature stimulus
response to topologically incorrect protein
defense response to virus
negative regulation of protein kinase activity
protein folding
negative regulation of kinase activity
response to endoplasmic reticulum stress
negative regulation of transferase activity
response to virus
endoplasmic reticulum lumen