UBA3

Entrez Summary: N/A
UniProt Summary: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:12740388, ECO:0000269|PubMed:9694792}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
BI-2536 0.001μM R01 exp41	-2.86
Hydroxychloroquine 30μM R08 exp490	-2.59
BI-6727 0.001μM R01 exp42	-2.55
NFN1 0.1μM R01 exp49	-2.52
Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53	-2.52
CAY10603 0.55μM R08 exp467	-2.5
Phorbol-12-myristate-13-acetate 0.57μM R08 exp514	-2.49
Lapatinib 5μM R01 exp47	-2.3
Nicotinamide 2000μM R01 exp50	-2.28
BS-181 20μM R08 exp461	-2.23
Daidzin 10μM R06 exp280	-2.17
Ethambutol 25μM R08 exp481	-2.17
YM155 0.0002μM R01 exp61	-2.17
Bafilomycin-A1 0.009μM R08 exp454	-2.11
RS-1 10μM R08 exp519	-2.08
B02 10μM R08 exp453	-2.07
4-Nitroquinoline-1-oxide 0.5μM R08 exp446	-2.02
Hypoxia 5%O2 R04 exp191	-1.99
UM131593 0.1μM R01 exp58	-1.97
GSK-J5 1.5μM R08 exp484	-1.95
PT-1 25μM R07 exp392	-1.91
YM155 0.001μM R08 exp432	-1.91
Nicotine 3000μM R08 exp510	-1.89
ETC-159 50μM R08 exp480	-1.88
Nocodazole 0.02μM R00 exp23	-1.88
Olaparib 4μM R08 exp512	-1.88
Ribavirin 10 to 15μM on day4 R04 exp160	-1.87
UMK57 0.6μM R03 exp107	-1.87
Zebularine 20μM R07 exp423	-1.82
LY2090314 0.003μM R08 exp499	-1.81
LY2090314 0.003μM R08 exp500 no dilution day6	-1.77
Cadmium 60μM R08 exp462	-1.74
Disulfiram 4.3μM R06 exp281	-1.73
Taxol 0.002μM R01 exp54	-1.73
Pyrazinamide 100μM R08 exp516	-1.72
Tanespimycin 14μM R08 exp527	1.77
Vitamin-D3 40μM R08 exp536	1.8
SNS-032 15μM R08 exp443	1.93
Staurosporine 0.02μM R08 exp524	1.94
Actinomycin-D 0.001μM R00 exp3	2.74
5-Fluorouracil 20μM R00 exp2	3.12

Global Fraction of Cell Lines Where Essential: 478/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	22/28
blood	16/28
bone	22/26
breast	23/33
central nervous system	32/56
cervix	4/4
colorectal	12/17
esophagus	8/13
fibroblast	1/1
gastric	9/16
kidney	15/21
liver	10/20
lung	49/75
lymphocyte	6/16
ovary	14/26
pancreas	20/24
peripheral nervous system	12/16
plasma cell	12/15
prostate	1/1
skin	9/24
soft tissue	5/9
thyroid	1/2
upper aerodigestive	20/22
urinary tract	18/29
uterus	4/5

Expression Distribution