UBA3

Entrez Summary: N/A
UniProt Summary: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:12740388, ECO:0000269|PubMed:9694792}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
BI-2536 0.001μM R01 exp41	-2.86
Hydroxychloroquine 30μM R08 exp490	-2.59
BI-6727 0.001μM R01 exp42	-2.55
NFN1 0.1μM R01 exp49	-2.52
Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53	-2.52
CAY10603 0.55μM R08 exp467	-2.5
Phorbol-12-myristate-13-acetate 0.57μM R08 exp514	-2.49
Lapatinib 5μM R01 exp47	-2.3
Nicotinamide 2000μM R01 exp50	-2.28
BS-181 20μM R08 exp461	-2.23
Daidzin 10μM R06 exp280	-2.17
Ethambutol 25μM R08 exp481	-2.17
YM155 0.0002μM R01 exp61	-2.17
Bafilomycin-A1 0.009μM R08 exp454	-2.11
RS-1 10μM R08 exp519	-2.08
B02 10μM R08 exp453	-2.07
4-Nitroquinoline-1-oxide 0.5μM R08 exp446	-2.02
Hypoxia 5%O2 R04 exp191	-1.99
UM131593 0.1μM R01 exp58	-1.97
GSK-J5 1.5μM R08 exp484	-1.95
PT-1 25μM R07 exp392	-1.91
YM155 0.001μM R08 exp432	-1.91
Nicotine 3000μM R08 exp510	-1.89
ETC-159 50μM R08 exp480	-1.88
Nocodazole 0.02μM R00 exp23	-1.88
Olaparib 4μM R08 exp512	-1.88
Ribavirin 10 to 15μM on day4 R04 exp160	-1.87
UMK57 0.6μM R03 exp107	-1.87
Zebularine 20μM R07 exp423	-1.82
LY2090314 0.003μM R08 exp499	-1.81
LY2090314 0.003μM R08 exp500 no dilution day6	-1.77
Cadmium 60μM R08 exp462	-1.74
Disulfiram 4.3μM R06 exp281	-1.73
Taxol 0.002μM R01 exp54	-1.73
Pyrazinamide 100μM R08 exp516	-1.72
Tanespimycin 14μM R08 exp527	1.77
Vitamin-D3 40μM R08 exp536	1.8
SNS-032 15μM R08 exp443	1.93
Staurosporine 0.02μM R08 exp524	1.94
Actinomycin-D 0.001μM R00 exp3	2.74
5-Fluorouracil 20μM R00 exp2	3.12

Global Fraction of Cell Lines Where Essential: 478/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	22/28
blood	16/28
bone	22/26
breast	23/33
central nervous system	32/56
cervix	4/4
colorectal	12/17
esophagus	8/13
fibroblast	1/1
gastric	9/16
kidney	15/21
liver	10/20
lung	49/75
lymphocyte	6/16
ovary	14/26
pancreas	20/24
peripheral nervous system	12/16
plasma cell	12/15
prostate	1/1
skin	9/24
soft tissue	5/9
thyroid	1/2
upper aerodigestive	20/22
urinary tract	18/29
uterus	4/5

Expression Distribution

NEDD8 activating enzyme activity
endomitotic cell cycle
NEDD8 transferase activity
protein neddylation
nuclear receptor binding
post-translational protein modification
protein heterodimerization activity
protein-containing complex
mitotic cell cycle
protein modification by small protein conjugation
protein modification by small protein conjugation or removal
identical protein binding
regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of nucleic acid-templated transcription
negative regulation of RNA biosynthetic process
proteolysis
negative regulation of RNA metabolic process
cell cycle
negative regulation of cellular macromolecule biosynthetic process
negative regulation of nucleobase-containing compound metabolic process
negative regulation of macromolecule biosynthetic process
ATP binding
negative regulation of cellular biosynthetic process
negative regulation of biosynthetic process
negative regulation of gene expression