NPRL3

Gene Information

Official Symbol: NPRL3
Official Name: NPR3 like, GATOR1 complex subunit
Aliases and Previous Symbols: N/A
Entrez ID: 8131
UniProt: Q12980
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The function of the encoded protein is not known. [provided by RefSeq, Aug 2011].
UniProt Summary: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway. {ECO:0000269|PubMed:23723238}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
LY2090314 0.003μM R08 exp500 no dilution day6	-4.93
LY2090314 0.003μM R08 exp499	-4.35
TRAIL 50ng/ml R00 exp36	-4.03
Golgicide-A 4μM R03 exp122	-3.71
Tubacin 1.6μM R07 exp416	-3.56
3-Bromopyruvate 7μM R04 exp175	-3.54
PU-H71 1μM R08 exp515	-3.4
Docosahexaenoic-acid 50μM R07 exp356	-3.39
Thimerosal 0.85μM R08 exp529	-3.36
CR131-b 0.005μM R08 exp474	-3.35
Dynasore 10μM R04 exp180	-3.34
Ixabepilone 0.004 to 0.005μM on day4 R04 exp184	-3.2
UM0011500 5μM R05 exp245	-3
Dynasore 7μM R08 exp436	-2.99
Artemisinin 50μM R08 exp450	-2.98
Docetaxel 0.001 to 0.002μM on day4 R04 exp159	-2.97
Quercetin 20μM R08 exp517	-2.96
UM0125461 0.74μM R02 exp84	-2.93
BVD-523 15μM R02 exp67	-2.92
Pimelic-diphenylamide-106 5μM R00 exp28	-2.82
TRAIL 5ng/ml R00 exp35	-2.82
Alpha-Amanitin 0.5μM R00 exp5	-2.75
Brefeldin A 0.02μM R00 exp8	-2.74
Bortezomib 0.005μM R00 exp6	-2.7
CCT251545 20μM R07 exp326	-2.66
BI-D1870 2μM R04 exp162	-2.6
Selumetinib 20μM R02 exp81	-2.58
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	-2.57
Hydroxyurea 100μM R07 exp329	-2.54
QNZ 0.01μM R08 exp439	-2.54
Genistein 15μM R07 exp360	-2.53
BN82002 4μM R07 exp340	-2.51
Combretastatin A4 0.002 to 0.003μM day4 R04 exp179	-2.48
Rifampicin 10μM R00 exp32	-2.47
Cryptotanshinone 12μM R05 exp227	-2.45
CB-5083 0.4μM R08 exp468	-2.42
Epothilone-B 0.0015μM R05 exp231	-2.4
Epigallocatechin gallate 20μM R05 exp230	-2.39
UMK57 0.6μM R03 exp107	-2.36
S-trityl-L-cysteine 0.5μM R05 exp243	-2.33
UM0011500 10μM R05 exp246	-2.32
Vincristine 0.0005μM R04 exp190	-2.32
Q15 2.7μM R02 exp79	-2.31
Bisphenol F 50μM R08 exp457	-2.3
JQ1 0.8μM R08 exp435	-2.26
5-Fluorouracil 20μM R00 exp2	-2.23
Bafilomycin-A1 0.009μM R08 exp454	-2.23
Mdivi-1 15μM R05 exp217	-2.22
SNS-032 15μM R08 exp443	-2.22
Clomiphene 4.4μM R02 exp68	-2.18
Q15 1 to 2μM on day4 R04 exp164	-2.18
All-trans-Retinoic-Acid 40μM R07 exp334	-2.16
Radicicol 0.16μM R05 exp242	-2.15
BML-284 0.09μM R08 exp460	-2.14
Nocodazole 0.02μM R00 exp23	-2.11
Epothilone-D 0.004 to 0.006μM on day4 R05 exp232	-2.1
Thalidomide 20μM R07 exp406	-2.1
A-395N 10μM R05 exp219	-2.08
MSC2530818 10μM R07 exp379	-2.08
2-Methoxyestradiol 0.55μM R07 exp310	-2.07
Citral 75μM R06 exp275	-2.07
UMK57 1μM R01 exp59	-2.03
Thapsigargin 0.005μM R07 exp407	-2.02
Bortezomib 0.05μM R00 exp7	-2
Rucaparib 6.5μM R08 exp520	-1.98
MLN-4924 0.2μM R00 exp21	-1.96
CoCl2 18μM R07 exp346	-1.95
Lenalidomide 20μM R07 exp375	-1.94
∆-9-Tetrahydrocannabinol 30μM R08 exp445	-1.94
Sulforaphane 9μM R08 exp526 no dilution day6	-1.93
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	-1.91
Mitomycin-C 0.06μM R08 exp503	-1.91
BI-D1870 3.15μM R02 exp66	-1.9
GSK-J4 1.5μM R08 exp441	-1.89
4-Nitroquinoline-1-oxide 0.5μM R08 exp446	-1.88
LLY-283 2.6μM R06 exp290	-1.88
Rotenone 2μM R00 exp33	-1.88
Vinblastine 0.002μM R01 exp60	-1.85
Rifampicin 30μM R08 exp430	-1.83
MK-1775 0.32μM R02 exp75	-1.8
Taxol 0.004μM R03 exp103	-1.8
Colchicine 0.009μM R05 exp215	-1.76
All-trans-Retinoic-Acid 8μM R07 exp333	-1.74
BH1 1μM R04 exp169	-1.74
Nocodazole 0.2μM R00 exp24	-1.73
Pterostilbene 16μM R02 exp78	-1.73
Aphidicolin 0.04μM R06 exp263	1.73
FTY720 3μM R08 exp483	1.75
A-395 10μM R05 exp218	1.76
5-Azacytidine 2μM R07 exp330	1.93
Tunicamycin 0.04 to 0.075μM on day4 R07 exp419	1.94
SGC0649 7μM R03 exp150	1.95
VER-155008 3.9μM R06 exp301	1.95
GSK343 3μM R03 exp124	2
NN-Dimethylsphingosine 2.5μM R08 exp508	2.02
Thioridazine 5μM R08 exp530	2.04
UNC1999 2μM R03 exp155	2.18
Tozasertib 0.1μM R07 exp414	2.24
SGC0946 7μM R03 exp151	2.42
Trientine 500μM R08 exp534	2.68
EPZ-5676 30μM R05 exp233	2.78
PF-06409577 20μM R07 exp389	2.93
Temozolomide 200μM R04 exp189	2.97
Erlotinib 10μM R05 exp216	3.01
Azithromycin 100μM R08 exp452	3.1
Hippuristanol 0.12μM R08 exp488	3.14
Phorbol-12-myristate-13-acetate 0.57μM R08 exp514	3.28
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	3.34
Hippuristanol 0.12μM R08 exp489 no dilution day6	4.2
Nicotine 3000μM R08 exp510	4.26
GSK626616 14μM R08 exp485	5.56
MK2206 4μM R08 exp504	7.53

Gene	Correlation
NPRL2	0.849
DEPDC5	0.839
SZT2	0.826
KPTN	0.729
ITFG2	0.722
C12orf66	0.713
SESN2	0.656
TSC2	0.653
TSC1	0.627
CCDC130	0.473
ZFP36L2	0.445
PRKAA1	0.411
ANP32A	0.409
HEXIM1	0.402

Essentiality in NALM6

Essentiality Rank: 14613
Expression level (log2 read counts): 5.36

Expression Distribution

GATOR1 complex
TORC1 signaling
aorta morphogenesis
TOR signaling
regulation of autophagosome assembly
regulation of vacuole organization
cellular response to amino acid starvation
response to amino acid starvation
negative regulation of TOR signaling
aorta development
artery morphogenesis
ventricular septum development
artery development
roof of mouth development
regulation of TOR signaling
cardiac septum development
cardiac ventricle development
cellular response to starvation
cardiac muscle tissue development
cardiac chamber development
regulation of macroautophagy
response to starvation
regulation of organelle assembly
cellular response to nutrient levels
cellular response to extracellular stimulus
GTPase activator activity
striated muscle tissue development
muscle tissue development
lysosomal membrane
regulation of autophagy
cellular response to external stimulus
blood vessel morphogenesis
positive regulation of GTPase activity
regulation of GTPase activity
blood vessel development
response to nutrient levels
negative regulation of intracellular signal transduction
vasculature development
cardiovascular system development
heart development