RPUSD3

Gene Information

Official Symbol: RPUSD3
Official Name: RNA pseudouridine synthase D3
Aliases and Previous Symbols: N/A
Entrez ID: 285367
UniProt: Q6P087
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a protein that functions in the assembly of the mitochondrial ribosome by adding a pseudouridine group to 16S rRNA. Loss of this gene results in causes defects in mitochondrial protein production. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2017].
UniProt Summary: Major enzyme responsible for the conversion of uridine to pseudouridine (PSI) at position 390 in mitochondrial COXI (MT- CO1) mRNA and at position 697-699 in mitochondrial COXIII (MT-CO3) mRNA, modulating the efficiency of mitochondrial protein synthesis without changes in transcript abundance or stability. {ECO:0000269|PubMed:27974379}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
CAY10603 0.55μM R08 exp467	1.73
Hydroxyurea 100μM R07 exp329	1.8
Mubritinib 0.2μM R01 exp48	1.91
GSK591 2.6μM R03 exp128	1.94
Chloroquine 32μM R08 exp470	1.97
UM0125461 0.74μM R02 exp84	2.05
Taxol 0.004μM R03 exp103	2.11
Oligomycin-A 2μM R00 exp25	2.62

Gene	Correlation
NDUFA3	0.623
GFM2	0.613
LARS2	0.612
QRSL1	0.61
NARS2	0.603
MRPS21	0.597
FTSJ2	0.593
NDUFAF5	0.587
PET112	0.587
NDUFS2	0.586
NDUFB11	0.584
GTPBP10	0.584
MRPL21	0.577
WBSCR16	0.577
GFM1	0.575
HSD17B10	0.573
MTRF1L	0.572
MALSU1	0.572
AARS2	0.571
NDUFA1	0.571
NDUFB6	0.57
FASTKD2	0.57
DAP3	0.565
NDUFAF7	0.564
MRPS34	0.564
CARS2	0.558
RARS2	0.557
DARS2	0.557
PARS2	0.553
YARS2	0.551
GRSF1	0.548
RBFA	0.546
NDUFA2	0.545
PET117	0.545
MRPS25	0.544
SELRC1	0.542
MARS2	0.542
RPUSD4	0.541
RTN4IP1	0.54
MRPS16	0.539
HMGXB3	0.539
C12orf65	0.537
MRPL23	0.534
ERAL1	0.531
HARS2	0.531
MTO1	0.531
MRPS26	0.53
NDUFB5	0.529
MCAT	0.529
METTL17	0.529
MRPL47	0.528
MRPL45	0.527
MRPL15	0.527
TFB1M	0.525
MRPS5	0.524
COA6	0.523
MTERFD1	0.521
MRPL53	0.521
NDUFC2	0.52
MRPS14	0.52
NSUN4	0.518
EARS2	0.518
PNPT1	0.513
MRPS27	0.512
NDUFA11	0.512
CHCHD1	0.511
NDUFAF4	0.508
MTIF2	0.508
TEFM	0.507
NDUFAF6	0.507
NDUFB9	0.506
VARS2	0.501
NDUFS5	0.501
MRPS12	0.5
TIMMDC1	0.5
MRPL49	0.498
ECSIT	0.498
ACAD9	0.497
MRP63	0.496
MRPS6	0.496
COA3	0.496
COX16	0.495
RNMTL1	0.495
TMEM261	0.494
TARS2	0.492
NDUFAF3	0.492
MTG1	0.488
MTG2	0.487
GATC	0.487
ATP5SL	0.486
SSBP1	0.485
NDUFA10	0.484
NDUFC1	0.483
PTCD3	0.483
MRPL20	0.482
TRUB2	0.481
RNASEH1	0.481
MRPL17	0.48
AIFM1	0.478
NDUFV1	0.476
NDUFS7	0.475
POLG2	0.474
MRPL39	0.473
MRPS7	0.473
FASTKD5	0.471
SLC25A26	0.47
MRPL43	0.47
MRPL28	0.468
DDX28	0.468
TUFM	0.467
NUBPL	0.465
MRPL3	0.463
NDUFB7	0.462
FARS2	0.462
FOXRED1	0.461
SCO2	0.46
GTPBP6	0.46
GLRX5	0.46
CLPP	0.46
MRPL51	0.459
PDSS1	0.457
POLRMT	0.455
KIAA0391	0.453
MRPL4	0.452
GTPBP8	0.447
TOMM6	0.447
NDUFS1	0.447
COQ2	0.444
COQ5	0.444
NDUFS3	0.442
TOMM20	0.442
SARS2	0.442
COX7B	0.44
COX15	0.439
MRPS33	0.438
MRPS22	0.435
ATP5C1	0.434
C1QBP	0.434
MTIF3	0.431
NDUFA8	0.43
MIPEP	0.43
NDUFA6	0.427
TFB2M	0.426
MRPL12	0.425
COX11	0.424
HIGD2A	0.423
TOMM22	0.423
NDUFB3	0.423
C17orf89	0.423
HEMK1	0.422
LRPPRC	0.422
NOA1	0.422
MRPS28	0.422
ATP5D	0.422
IBA57	0.421
BCS1L	0.42
MRPS18A	0.419
MRPS11	0.419
MTRF1	0.419
XPNPEP3	0.417
MRPS35	0.416
MRPS10	0.416
MRPL18	0.415
SFXN4	0.415
POLG	0.413
MRPS24	0.413
SUPV3L1	0.412
NDUFAF1	0.411
PREPL	0.411
MRPL22	0.411
NDUFB10	0.411
MRPL44	0.41
NDUFB4	0.409
SLC25A3	0.409
WARS2	0.408
C10orf2	0.407
MRPL55	0.405
COX19	0.404
COQ7	0.402
MRPS23	0.402
TIMM21	0.401
MTERFD2	0.401
COX17	0.401
ICT1	0.4

Global Fraction of Cell Lines Where Essential: 7/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	1/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	1/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	2/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 1835
Expression level (log2 read counts): 5.24

Expression Distribution

pseudouridine synthase activity
positive regulation of mitochondrial translation
pseudouridine synthesis
regulation of mitochondrial translation
regulation of mitochondrial gene expression
positive regulation of translation
positive regulation of cellular amide metabolic process
RNA modification
regulation of translation
mitochondrial matrix
regulation of cellular amide metabolic process
mRNA processing
posttranscriptional regulation of gene expression
mRNA metabolic process
RNA processing
RNA binding
positive regulation of cellular protein metabolic process
RNA metabolic process
positive regulation of protein metabolic process
positive regulation of macromolecule biosynthetic process
positive regulation of cellular biosynthetic process
positive regulation of gene expression
gene expression
positive regulation of biosynthetic process