NAE1

Gene Information

Official Symbol: N/AE1
Official Name: NEDD8 activating enzyme E1 subunit 1
Aliases and Previous Symbols: N/A
Entrez ID: 8883
UniProt: Q13564
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:10722740, ECO:0000269|PubMed:12740388}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
1-Methyl-nicotinamide-chloride 1000μM R03 exp113	-2.52
Cidofovir 10μM R07 exp345	-2.47
BRD2 inhibitor II 20μM R07 exp341	-2.21
Phenformin 20μM R03 exp143	-2.01
Pimelic-diphenylamide-106 0.5μM R00 exp27	-1.94
Methotrexate 0.01μM R08 exp501	-1.92
BAY-598 4μM R05 exp220	-1.86
Parthenolide 2.5μM R05 exp238	-1.84
Adefovir 20μM R07 exp332	-1.75
Tanespimycin 14μM R08 exp527	1.83
Pimelic-diphenylamide-106 5μM R00 exp28	2.15
Milciclib 2μM R08 exp502	2.16
Staurosporine 0.02μM R08 exp524	2.72
5-Fluorouracil 20μM R00 exp2	2.95
Actinomycin-D 0.001μM R00 exp3	3.05

Gene	Correlation
UBE2M	0.634
UBA3	0.632
THG1L	0.626
GFPT1	0.619
RTCB	0.608
PMM2	0.604
RNMT	0.591
GMPPB	0.589
TRIAP1	0.586
UAP1	0.581
RPP21	0.579
ALG1	0.576
HARS	0.576
DPAGT1	0.574
ADSL	0.572
HSPD1	0.555
SRP14	0.544
RPTOR	0.539
UHRF1	0.535
TRMT5	0.526
DHPS	0.525
AARS	0.524
MED16	0.522
ALG13	0.521
GNB1L	0.516
EXOC4	0.516
GEMIN5	0.516
EIF4E	0.512
VEZT	0.511
GTF3C3	0.509
EPRS	0.507
OSGEP	0.507
GTF3C2	0.505
TTI2	0.504
CCT7	0.503
PELO	0.501
CBFB	0.498
SEC61G	0.498
ATXN10	0.498
ALG2	0.498
GTF3C4	0.496
DR1	0.493
UBE2L3	0.493
TRNT1	0.493
OTUD5	0.493
VRK1	0.491
MEAF6	0.488
BAP1	0.486
NSMCE1	0.485
SEH1L	0.481
UTP23	0.48
WDR59	0.48
TEN1	0.479
PAM16	0.479
DDX20	0.479
ATP5J	0.478
CEP57	0.477
GEMIN8	0.477
PLAA	0.477
ALG14	0.476
GTF3C6	0.476
ENY2	0.474
GEMIN6	0.471
HCCS	0.47
PPCS	0.47
RABGGTB	0.47
DOHH	0.469
HAUS8	0.468
CCDC101	0.468
LAMTOR5	0.467
PPP6C	0.465
ATM	0.463
TAZ	0.463
EXOC3	0.462
TBCA	0.461
YARS	0.46
TELO2	0.459
POLR3H	0.459
RAD1	0.458
GLMN	0.456
APAF1	0.456
CARS	0.455
WDR24	0.454
VHL	0.454
TAMM41	0.454
FAF2	0.454
NBN	0.453
TSEN54	0.453
BAK1	0.452
CSNK2B	0.452
CHCHD4	0.451
GTF3A	0.451
TXNRD1	0.451
MED23	0.45
DDOST	0.45
TPRKB	0.449
UBE2K	0.447
DDX6	0.447
SPCS2	0.447
NUP50	0.443
DDX55	0.443
CASP9	0.441
PIK3R4	0.441
PPP4C	0.44
TTC4	0.44
ALG5	0.44
TMEM41B	0.439
TSEN34	0.438
SRP68	0.438
TTI1	0.437
TLE4	0.436
ORAOV1	0.436
ENO1	0.434
DOLK	0.434
TXNL4B	0.433
MED25	0.433
DAD1	0.433
RPE	0.432
TGS1	0.43
UXT	0.43
MTOR	0.43
TRIM28	0.429
POLR3B	0.428
FH	0.426
SPCS3	0.426
UBE2G2	0.425
DHDDS	0.425
ATP5B	0.424
UBQLN1	0.424
NARS	0.424
PHB	0.424
PGGT1B	0.423
MLLT1	0.422
GTF2A2	0.421
MED4	0.42
ZRSR2	0.42
FNTA	0.419
RAD50	0.419
TSEN2	0.417
ALG6	0.417
PPP2R3C	0.417
ACTR10	0.417
ATIC	0.416
CDIPT	0.415
PMPCB	0.414
ROMO1	0.414
TBP	0.413
CTC1	0.411
LSM10	0.41
MARCH5	0.41
DCTN6	0.41
DDX1	0.409
CRLS1	0.409
PPA2	0.408
TBCB	0.408
CTDNEP1	0.406
PISD	0.406
CEP350	0.404
BTAF1	0.403
TIMM13	0.403
WARS	0.402
GEMIN7	0.402
XRCC2	0.401
SLC25A26	0.4

Global Fraction of Cell Lines Where Essential: 498/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	18/28
blood	13/28
bone	25/26
breast	22/33
central nervous system	33/56
cervix	3/4
colorectal	14/17
esophagus	9/13
fibroblast	1/1
gastric	6/16
kidney	12/21
liver	9/20
lung	57/75
lymphocyte	6/16
ovary	17/26
pancreas	18/24
peripheral nervous system	10/16
plasma cell	8/15
prostate	1/1
skin	17/24
soft tissue	5/9
thyroid	0/2
upper aerodigestive	17/22
urinary tract	22/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 1248
Expression level (log2 read counts): 6.58

Expression Distribution

NEDD8 activating enzyme activity
mitotic DNA replication checkpoint
protein neddylation
DNA replication checkpoint
mitotic G2/M transition checkpoint
neuron apoptotic process
neuron death
negative regulation of G2/M transition of mitotic cell cycle
negative regulation of cell cycle G2/M phase transition
mitotic DNA integrity checkpoint
DNA integrity checkpoint
mitotic cell cycle checkpoint
cell cycle checkpoint
regulation of G2/M transition of mitotic cell cycle
regulation of neuron apoptotic process
regulation of cell cycle G2/M phase transition
negative regulation of mitotic cell cycle phase transition
negative regulation of cell cycle phase transition
ubiquitin protein ligase binding
negative regulation of mitotic cell cycle
regulation of neuron death
negative regulation of cell cycle process
post-translational protein modification
regulation of mitotic cell cycle phase transition
regulation of cell cycle phase transition
protein heterodimerization activity
negative regulation of cell cycle
protein-containing complex
mitotic cell cycle process
regulation of mitotic cell cycle
mitotic cell cycle
regulation of cell cycle process
protein modification by small protein conjugation
apoptotic process
protein modification by small protein conjugation or removal
cell cycle process
programmed cell death
cell death
regulation of cell cycle
cell cycle