CHAF1A

Gene Information

Official Symbol: CHAF1A
Official Name: chromatin assembly factor 1 subunit A
Aliases and Previous Symbols: N/A
Entrez ID: 10036
UniProt: Q13111
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). {ECO:0000250, ECO:0000269|PubMed:15327775}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
HMS-I1 10μM R06 exp256	-2.33
Etoposide 10μM R00 exp20	-2.16
Mitomycin-C 0.06μM R08 exp503	-2.15
RS-1 10μM R08 exp519	-2.03
Oligomycin-A 20μM R00 exp26	-1.91
SB743921 0.001μM R05 exp244	-1.85
DABN 2μM R00 exp16	1.7
BH1 1μM R04 exp169	1.72
A-395 10μM R05 exp218	1.74
Lenalidomide 20μM R07 exp375	1.76
Bafilomycin-A1 0.009μM R08 exp454	1.79
Chloramphenicol 2μM R00 exp12	1.83
Cincreasin 100μM R08 exp473	1.83
BML-284 0.09μM R08 exp460	1.85
Lead acetate 990μM R08 exp496	1.87
Dimethyloxaloylglycine 100μM R05 exp229	1.88
Quercetin 20μM R08 exp517	1.88
Apcin 50 to 100μM on day4 R04 exp176	1.92
THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412	1.93
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	1.94
THZ531 0.11μM R07 exp409	1.94
Doxycycline 10μM R00 exp18	1.98
ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319	1.99
Methotrexate 0.01μM R08 exp501	2
CCCP 1μM R00 exp11	2.02
THZ531 0.11 to 0.175μM on day4 R07 exp413	2.1
CVT-10216 0.1μM R06 exp278	2.11
NN-Diethyl-meta-toluamide 100μM R05 exp237	2.11
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	2.12
Trametinib 10μM R02 exp83	2.15
Selumetinib 20μM R02 exp81	2.32
UNC1999 2μM R03 exp155	2.32
THZ531 0.11 to 0.125μM on day4 R07 exp410	2.33
Dexamethasone 0.006μM R07 exp351	2.57
CCCP 0.1μM R00 exp10	3.14

Gene	Correlation
TCEB3C	0.581
PSMB4	0.57
TCEB3CL2	0.55
TCEB3CL	0.549
TICRR	0.543
VASN	0.535
TBC1D3F	0.535
CCNA2	0.528
RPS19BP1	0.518
PSMB2	0.514
ZNF830	0.509
ANKLE2	0.509
SNRPA1	0.502
COPE	0.5
TBC1D3H	0.498
TBC1D3G	0.494
COPG1	0.493
C1QTNF9B	0.493
NUP153	0.493
TFIP11	0.492
VPS28	0.489
IK	0.487
UPF1	0.487
EXOSC9	0.484
AQR	0.48
RAD51	0.48
POLR2J3	0.48
CBWD3	0.479
SLC4A1	0.477
PDCD11	0.476
RPL35	0.473
HIST1H2BE	0.471
TBC1D3	0.471
CHD4	0.47
CBWD5	0.47
IGFN1	0.469
PNO1	0.468
COPB1	0.468
PRIM1	0.468
PSMC1	0.467
PSMC2	0.465
CACTIN	0.462
NUP160	0.462
TYMS	0.462
LRR1	0.46
SUPT5H	0.46
NUPL1	0.459
KCTD17	0.459
XYLT2	0.458
SLC6A17	0.458
PRPF8	0.457
DOCK6	0.455
ARCN1	0.455
RPS5	0.454
SEC13	0.454
GAR1	0.454
RRP7A	0.454
TBC1D3I	0.453
ATP6V1C1	0.452
DIDO1	0.452
PSMA6	0.452
PSMD7	0.452
TBC1D3E	0.452
PSMD3	0.451
TBC1D3K	0.451
TBC1D3L	0.451
MT1E	0.45
PAN3	0.45
NOL12	0.449
GOLGA6L3	0.449
TPX2	0.447
POLE	0.447
DTL	0.446
EIF4A3	0.446
CNOT1	0.446
PSMC5	0.443
EXD1	0.443
CDH24	0.443
CTAGE4	0.442
RIOK2	0.44
ZNF574	0.438
EXOSC5	0.438
ATP6V1B2	0.437
CDCA3	0.437
TSG101	0.437
MED29	0.437
LOC643669	0.437
SSH2	0.436
CBWD6	0.436
AAMP	0.436
C14orf93	0.436
ISCU	0.436
TBC1D3C	0.435
PAF1	0.434
ANKLE1	0.434
ORC1	0.433
GOLGA8O	0.432
RPS2	0.432
RPS19	0.431
GOLGA6L1	0.431
HIST2H3C	0.431
HIST2H3A	0.431
SART1	0.431
UTP3	0.43
PRPF19	0.43
CWC22	0.43
NAPA	0.43
ATP6V1A	0.429
FAM160A1	0.429
NPIPB5	0.429
PHF2	0.428
CCDC116	0.428
RFC1	0.428
FBXW9	0.428
PSMB3	0.427
PSMA7	0.427
RPS28	0.427
CDC73	0.426
XAB2	0.426
RPS12	0.426
DNM2	0.425
DHX38	0.424
NUFIP2	0.424
CGB2	0.424
SF3A1	0.423
MYO1H	0.422
S1PR4	0.421
DDTL	0.421
KRT78	0.421
COL2A1	0.42
RPS3	0.419
DNTTIP2	0.419
GOLGA6L4	0.419
ZNF655	0.418
SNRNP200	0.418
FAM231C	0.417
KIF3C	0.417
CHCHD3	0.417
SAP30BP	0.417
FANCM	0.417
FAM231A	0.417
RBM39	0.417
SKIV2L2	0.416
INTS5	0.415
MBTD1	0.415
NOL10	0.415
POLA1	0.415
RAD21	0.414
ATP6AP1	0.414
CTAGE9	0.414
ACSF2	0.414
HIVEP3	0.413
GOLGA6A	0.413
TAGLN	0.411
AATF	0.411
CDC6	0.411
RRP15	0.41
NARFL	0.409
RPL7L1	0.409
OR10G3	0.409
RPL37A	0.408
ATP6AP2	0.408
SPECC1L	0.408
HIST1H4B	0.407
RPL30	0.407
SF3A3	0.407
TRAPPC11	0.407
CDC45	0.406
GID4	0.406
OSBP	0.405
PPFIA4	0.405
PSMC3	0.405
SCGB1C1	0.405
ZFAND5	0.404
PDE4DIP	0.404
SH3BP1	0.404
EIF6	0.403
SNX15	0.402
CCDC64B	0.401
POLR2A	0.401
EXOSC3	0.401
POLR2D	0.4
SEMA6B	0.4
STIL	0.4
TONSL	0.4
EGR2	0.4
PSMD11	0.4

Global Fraction of Cell Lines Where Essential: 667/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	25/28
blood	23/28
bone	24/25
breast	31/33
central nervous system	55/56
cervix	4/4
colorectal	16/17
esophagus	12/13
fibroblast	1/1
gastric	14/15
kidney	17/21
liver	15/20
lung	68/75
lymphocyte	11/14
ovary	25/26
pancreas	23/24
peripheral nervous system	14/16
plasma cell	14/15
prostate	1/1
skin	24/24
soft tissue	7/7
thyroid	1/2
upper aerodigestive	17/22
urinary tract	24/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 128
Expression level (log2 read counts): 7.45

Expression Distribution

CAF-1 p150
CAF1A

CAF-1 complex
chromo shadow domain binding
DNA replication-dependent nucleosome organization
DNA replication-dependent nucleosome assembly
nucleosome assembly
unfolded protein binding
chromatin assembly
chromatin assembly or disassembly
nucleosome organization
DNA packaging
protein-DNA complex assembly
DNA replication
nuclear chromatin
protein-DNA complex subunit organization
DNA conformation change
chromatin binding
DNA repair
protein-containing complex
chromatin organization
DNA metabolic process
cellular response to DNA damage stimulus
cellular protein-containing complex assembly
chromosome organization
identical protein binding
cell cycle
protein-containing complex assembly
cellular response to stress
cellular macromolecule biosynthetic process
macromolecule biosynthetic process
protein-containing complex subunit organization