PAF1

Gene Information

Official Symbol: PAF1
Official Name: PAF1 homolog, Paf1/RNA polymerase II complex component
Aliases and Previous Symbols: N/A
Entrez ID: 54623
UniProt: Q8N7H5
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro. {ECO:0000269|PubMed:16491129, ECO:0000269|PubMed:19410543, ECO:0000269|PubMed:19952111, ECO:0000269|PubMed:20178742, ECO:0000269|PubMed:20541477, ECO:0000269|PubMed:21329879, ECO:0000269|PubMed:22419161}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Etoposide 1μM R00 exp19	-2.15
BI-6727 0.04μM R03 exp98	-2.06
Nocodazole 0.1μM R03 exp94	-1.99
Ammonium tetrathiomolybdate 10μM R08 exp448	-1.89
Vinblastine 0.015μM R05 exp208	-1.74
Actinomycin-D 0.001μM R00 exp3	-1.72
Cholesterol 0.003 to 0.006 to 0.1μM on day2 then day6 R08 exp471	1.7
39°C R06 exp303	1.71
All-trans-Retinoic-Acid 8μM R07 exp333	1.72
CCCP 0.1μM R00 exp10	1.72
Selumetinib 20μM R02 exp81	1.73
Rapamycin 2μM plus FK-506 5μM R07 exp308	1.73
Golgicide-A 2μM R03 exp121	1.75
SGC0946 7μM R03 exp151	1.76
Beta-Estradiol 0.55μM R07 exp425	1.77
NFN1 0.1μM R01 exp49	1.85
Nocodazole 0.02μM R00 exp23	1.85
CCT251545 20μM R07 exp326	1.87
Tenofovir 10μM R07 exp405	1.88
Wortmannin 0.5μM R00 exp37	1.89
Senexin-A 25μM R07 exp400	1.9
TRAIL 5ng/ml R00 exp35	1.9
A-395 10μM R05 exp218	1.91
Chloramphenicol 2μM R00 exp12	1.91
IWR1 50μM R08 exp495	1.94
Ribavirin 10μM R01 exp52	1.94
Thalidomide 20μM R07 exp406	1.96
Rapamycin 10μM R00 exp30	2.04
GSK591 2.6μM R03 exp128	2.06
FK-506 5μM R07 exp358	2.14
SB203580 25μM R03 exp149	2.23
Talazoparib 0.006μM R06 exp299	2.28
5-Fluorouracil 2μM R00 exp1	2.41
2-Methoxyestradiol 0.2μM R01 exp40	2.76
ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319	2.95

Gene	Correlation
PSMB4	0.567
GOLGA6L1	0.556
TCEB3CL2	0.543
CTR9	0.537
CHD4	0.534
CDC73	0.533
VASN	0.523
PAN3	0.516
TCEB3C	0.516
KRT78	0.509
C14orf93	0.505
HIST2H3A	0.501
ORC1	0.5
HIST2H3C	0.5
NARFL	0.497
TCEB3CL	0.493
SF3A3	0.493
RPS9	0.493
PSMB3	0.492
EXOSC9	0.49
PSMD1	0.489
CDCA3	0.487
NPIPB5	0.487
COPE	0.483
PSMC2	0.483
ZNF106	0.482
ZFAND5	0.482
CEP192	0.48
ANKLE2	0.478
SNRNP70	0.478
DIDO1	0.477
IK	0.477
COPB1	0.475
SMG7	0.475
SF3A1	0.474
MT1E	0.472
MYO1H	0.472
POLE	0.471
CKAP5	0.47
ARCN1	0.469
GOLGA6L6	0.468
DDTL	0.467
RPS2	0.466
TSG101	0.464
ORC6	0.463
COPG1	0.462
RBM39	0.461
RRP15	0.459
GOLGA8O	0.458
CCNA2	0.458
TBC1D3G	0.457
PSMA6	0.456
TBC1D3H	0.456
ZNF148	0.455
POLA1	0.455
PSMC5	0.454
SNRPA1	0.453
ISCU	0.452
NBPF10	0.451
UPF1	0.451
NOP16	0.45
C1QTNF9B	0.449
XYLT2	0.449
EIF4A3	0.448
PSMD11	0.448
POLR2J3	0.447
AAMP	0.446
LRR1	0.446
SUPT6H	0.445
PSMD7	0.444
POLA2	0.444
INTS5	0.443
PSMD3	0.443
ADRM1	0.443
TICRR	0.443
RPL35	0.442
RPS3	0.441
ZNF830	0.441
NUPL1	0.441
PAN2	0.441
SLC4A1	0.44
SUPT5H	0.44
RPS19	0.439
PDE4DIP	0.439
PSMD14	0.439
ATP6V0C	0.439
CCDC116	0.439
EXOSC6	0.439
UQCRH	0.438
TBC1D3I	0.438
SETD1A	0.437
MYT1L	0.437
TBC1D3E	0.437
PWP2	0.437
TBC1D3K	0.436
PSMC6	0.436
NUP205	0.436
TBC1D3L	0.436
CHAF1A	0.434
NCL	0.432
SAP30BP	0.432
TFIP11	0.432
DHFR	0.431
DTL	0.431
BOD1L1	0.43
RPS12	0.43
HIST1H4B	0.429
PSMB2	0.429
NUP160	0.428
SF3B2	0.428
RAD21	0.428
CLPX	0.427
COPA	0.427
NPIPB8	0.427
ABHD8	0.426
CGB2	0.426
RRN3	0.426
TBC1D3C	0.424
ISY1	0.424
RPL6	0.424
RPS19BP1	0.424
PDCD11	0.423
HIST2H2BF	0.423
WDR75	0.422
NOL12	0.422
CWC22	0.422
PCF11	0.421
RRP7A	0.421
RPL38	0.42
HIVEP3	0.42
ZNF655	0.42
CDC6	0.419
TAF15	0.419
GON4L	0.419
COL2A1	0.418
CLN6	0.418
RPSA	0.418
SSH2	0.417
CBWD3	0.416
EXOSC5	0.415
AQR	0.415
FAM231C	0.415
FAM231A	0.415
PSMC1	0.415
POLR2A	0.415
MYO18A	0.413
TAGLN	0.412
SLC6A17	0.412
SCAF1	0.41
TBC1D3F	0.41
NHP2L1	0.409
DDT	0.409
CNOT1	0.408
COPB2	0.407
BRD2	0.406
TOP1MT	0.406
CBWD5	0.406
RIOK2	0.405
CDT1	0.405
MYB	0.405
RPS28	0.404
OSBP	0.404
FBXW9	0.403
NDC80	0.403
PSAP	0.403
PLK4	0.402
SEC13	0.401
RNPC3	0.401
CBWD6	0.401
RPS5	0.4
RPL7L1	0.4
SNIP1	0.4
TRIM52	0.4

Global Fraction of Cell Lines Where Essential: 214/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	0/1
bile duct	8/28
blood	8/28
bone	12/25
breast	17/33
central nervous system	13/56
cervix	1/4
colorectal	3/17
esophagus	0/13
fibroblast	1/1
gastric	1/15
kidney	6/21
liver	6/20
lung	18/75
lymphocyte	7/14
ovary	13/26
pancreas	4/24
peripheral nervous system	6/16
plasma cell	7/15
prostate	0/1
skin	5/24
soft tissue	2/7
thyroid	1/2
upper aerodigestive	4/22
urinary tract	7/29
uterus	3/5

Essentiality in NALM6

Essentiality Rank: 655
Expression level (log2 read counts): 5.95

Expression Distribution

Cdc73/Paf1 complex
histone H2B ubiquitination
positive regulation of mRNA 3-end processing
endodermal cell fate commitment
nucleosome positioning
positive regulation of transcription elongation from RNA polymerase II promoter
transcriptionally active chromatin
mRNA polyadenylation
cell fate commitment involved in formation of primary germ layer
regulation of mRNA 3-end processing
histone monoubiquitination
RNA polyadenylation
regulation of transcription elongation from RNA polymerase II promoter
positive regulation of mRNA processing
positive regulation of DNA-templated transcription, elongation
RNA polymerase II complex binding
positive regulation of histone methylation
histone ubiquitination
endodermal cell differentiation
endoderm formation
positive regulation of cell cycle G1/S phase transition
regulation of DNA-templated transcription, elongation
protein monoubiquitination
regulation of histone methylation
endoderm development
transcription elongation from RNA polymerase II promoter
negative regulation of myeloid cell differentiation
positive regulation of mRNA metabolic process
mRNA 3-end processing
DNA-templated transcription, elongation
positive regulation of histone modification
positive regulation of cell cycle phase transition
positive regulation of chromatin organization
formation of primary germ layer
chromatin assembly
RNA 3-end processing
stem cell population maintenance
negative regulation of hemopoiesis
maintenance of cell number
regulation of mRNA processing