RPUSD4

Gene Information

Official Symbol: RPUSD4
Official Name: RNA pseudouridine synthase D4
Aliases and Previous Symbols: N/A
Entrez ID: 84881
UniProt: Q96CM3
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Converts specific uridines to pseudouridine (PSI) in different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome (PubMed:27974379). Acts on position 39 in mitochondrial tRNA(Phe) (PubMed:28082677). {ECO:0000269|PubMed:27974379, ECO:0000269|PubMed:28082677}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Lapatinib 5μM R01 exp47	-1.76
Hypoxia 5%O2 R04 exp191	-1.73
Milciclib 2μM R08 exp502	1.71
CR131-b 0.005μM R08 exp474	1.76
Torin1 0.08μM R02 exp82	1.76
Ammonium tetrathiomolybdate 10μM R08 exp448	1.79
Olaparib 4μM R08 exp512	1.86
Oligomycin-A 2μM R00 exp25	1.88
QNZ 0.01μM R05 exp241	1.88
TIC10 10μM R08 exp532	1.91
Arsenate 40μM R06 exp264	1.95
Monensin 0.3μM R08 exp507	2.01
ABT-702 5μM plus Deferoxamine 11μM R07 exp321	2.06
CI-1040 9.5μM R08 exp472	2.09
BI-D1870 3.15μM R02 exp66	2.17
Hinokiflavone 12μM R08 exp487	2.17
Genistein 15μM R07 exp360	2.19
PU-H71 1μM R08 exp515	2.2
Hydroxyurea 100μM R07 exp329	2.26
Thimerosal 0.85μM R08 exp529	2.3
Tanespimycin 14μM R08 exp527	2.34
Aphidicolin 0.4μM R08 exp440	2.38
Vemurafenib 6.6μM R08 exp434	2.43
UM0125461 0.74μM R02 exp84	2.79
GSK-J4 1.5μM R08 exp441	2.85

Gene	Correlation
MRPL21	0.738
YARS2	0.73
SELRC1	0.729
HSD17B10	0.701
TARS2	0.697
MRPS6	0.69
NARS2	0.689
MRPL23	0.688
MRPS16	0.681
MRPS21	0.678
VARS2	0.675
DAP3	0.675
MRPS5	0.666
GFM1	0.663
MRPS34	0.661
MRPS14	0.661
FASTKD5	0.654
KIAA0391	0.652
PARS2	0.649
RBFA	0.647
RNASEH1	0.645
SCO2	0.645
QRSL1	0.643
C12orf65	0.642
COX16	0.642
SARS2	0.64
MRP63	0.636
MRPS18A	0.635
GFM2	0.635
LARS2	0.633
DARS2	0.633
MRPS26	0.63
GTPBP10	0.622
EARS2	0.622
FTSJ2	0.621
MTG2	0.621
BCS1L	0.619
MRPL47	0.616
MRPL53	0.614
RARS2	0.614
FARS2	0.612
MARS2	0.606
MTPAP	0.603
MRPL12	0.602
GATC	0.599
MRPS24	0.599
TEFM	0.598
TUFM	0.597
SUPV3L1	0.596
MRPL20	0.595
COA3	0.594
SLC25A26	0.592
MRPS2	0.585
PDSS1	0.582
NDUFA3	0.581
PET112	0.581
MTRF1L	0.58
MRPS25	0.576
NDUFB11	0.575
MRPL55	0.573
C17orf89	0.572
C10orf2	0.571
COA6	0.57
COQ2	0.57
MRPS23	0.563
PNPT1	0.562
MRPS11	0.561
AARS2	0.559
TRUB2	0.553
POLG2	0.552
MRPL15	0.551
UQCRC2	0.55
TOMM20	0.548
MRPS12	0.547
COX15	0.546
COX7B	0.544
NDUFAF7	0.543
MTERFD2	0.542
WBSCR16	0.541
RPUSD3	0.541
COX17	0.538
MRPS9	0.538
MRPS22	0.537
MRPL3	0.536
COX6C	0.535
MRPS10	0.534
POLG	0.528
MRPL44	0.528
HMGXB3	0.527
TMEM242	0.527
CHCHD4	0.527
MRPL4	0.526
MRPS7	0.524
MTG1	0.524
TIMM8B	0.522
MRPL45	0.517
MRPL34	0.516
NDUFAF3	0.516
COX11	0.516
MRPL18	0.513
HARS2	0.513
ATP5C1	0.512
PET117	0.511
METTL17	0.511
NDUFA2	0.511
CARS2	0.509
AASDHPPT	0.509
MRPS35	0.508
NDUFC2	0.506
MRPS33	0.505
RPIA	0.504
PGM3	0.503
IBA57	0.502
ELAC2	0.5
TOMM22	0.499
MTIF3	0.498
HCCS	0.498
ATP5D	0.498
DHRSX	0.497
MIPEP	0.496
UQCRQ	0.496
MRPL39	0.494
CHCHD1	0.493
MCAT	0.492
LRPPRC	0.492
NDUFB5	0.49
MRPL11	0.489
DHX30	0.488
NDUFA1	0.487
ECSIT	0.487
MRPL41	0.486
MRPL36	0.485
TRIAP1	0.485
DNLZ	0.484
WARS2	0.483
COQ6	0.483
MRPL43	0.482
NDUFS7	0.481
RNMTL1	0.481
MRPL46	0.48
NDUFB3	0.478
UQCC2	0.478
LSM10	0.477
MALSU1	0.476
TMEM261	0.475
NDUFA11	0.474
COQ4	0.473
MTERFD1	0.472
OPA1	0.471
MRPL1	0.471
COA5	0.47
MRPL33	0.47
MRPL22	0.469
COQ3	0.468
TOMM6	0.468
NDUFAF5	0.467
ATP5SL	0.466
RTN4IP1	0.465
MRPL2	0.464
CRLS1	0.464
ATP5J	0.464
POLRMT	0.463
UQCR10	0.46
PMM2	0.459
ATP5B	0.459
NDUFV1	0.458
SSBP1	0.457
TPK1	0.456
AIFM1	0.456
TFB1M	0.455
MRPL27	0.454
MRPL13	0.453
MINOS1	0.452
COQ7	0.451
TFAM	0.451
PPCS	0.449
HIGD2A	0.447
COX5A	0.447
MRPS27	0.446
NFU1	0.445
ROMO1	0.445
NDUFS2	0.442
NDUFB9	0.441
NDUFB6	0.441
NDUFAF4	0.439
TAMM41	0.438
PET100	0.438
FH	0.438
ERAL1	0.437
TIMM17A	0.435
MRPL52	0.434
TMEM126B	0.433
TIMMDC1	0.432
PTCD1	0.431
MRPS17	0.429
FASTKD2	0.429
CYCS	0.428
MRPL42	0.428
ATXN10	0.427
MTO1	0.425
TOMM40	0.425
MRPL35	0.425
ATP5E	0.423
PTDSS1	0.422
ATP5A1	0.421
DDX28	0.421
GLRX5	0.421
MRPL49	0.42
SLC25A19	0.42
COX6B1	0.418
PRKRIR	0.418
TRMT10A	0.418
ISCA2	0.417
SRD5A3	0.416
PTRHD1	0.416
MRPL17	0.415
ZRSR2	0.412
RPE	0.412
MRPS18B	0.411
GTPBP6	0.41
TBRG4	0.409
GRSF1	0.409
PPA2	0.409
DPAGT1	0.408
VEZT	0.408
ATP5O	0.408
MRPL51	0.407
ALAS1	0.407
ALDH18A1	0.407
TAZ	0.405
ACAD9	0.405
ALG8	0.404
NDUFS5	0.403
TRMT61A	0.403
THG1L	0.403
TOMM7	0.402
DOHH	0.402
MRPL37	0.401

Global Fraction of Cell Lines Where Essential: 22/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	2/33
central nervous system	1/56
cervix	0/4
colorectal	1/17
esophagus	1/13
fibroblast	0/1
gastric	1/16
kidney	1/21
liver	0/20
lung	2/75
lymphocyte	1/16
ovary	0/26
pancreas	1/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	2/24
soft tissue	1/9
thyroid	0/2
upper aerodigestive	2/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 623
Expression level (log2 read counts): 6.3

Expression Distribution

pseudouridine synthase activity
positive regulation of mitochondrial translation
pseudouridine synthesis
regulation of mitochondrial translation
regulation of mitochondrial gene expression
positive regulation of translation
tRNA processing
positive regulation of cellular amide metabolic process
RNA modification
tRNA metabolic process
regulation of translation
mitochondrial matrix
ncRNA processing
regulation of cellular amide metabolic process
ncRNA metabolic process
posttranscriptional regulation of gene expression
RNA processing
RNA binding
positive regulation of cellular protein metabolic process
RNA metabolic process
positive regulation of protein metabolic process
positive regulation of macromolecule biosynthetic process
positive regulation of cellular biosynthetic process
positive regulation of gene expression
gene expression
positive regulation of biosynthetic process