MED16

Gene Information

Official Symbol: MED16
Official Name: mediator complex subunit 16
Aliases and Previous Symbols: N/A
Entrez ID: 10025
UniProt: Q9Y2X0
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269|PubMed:10198638, ECO:0000269|PubMed:10235266}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	-2.4
Rapamycin 0.001μM R08 exp429	-2
Dimethyloxaloylglycine 100μM R05 exp229	-1.9
Tenofovir 10μM R07 exp405	-1.85
NN-Dimethylsphingosine 2.5μM R08 exp508	-1.84
Nicotinamide 2000μM R01 exp50	-1.81
VER-155008 3.9μM R06 exp301	-1.75
All-trans-Retinoic-Acid 40μM R07 exp334	-1.7
CI-1040 9.5μM R08 exp472	1.9
Doxorubicin 0.02μM R08 exp478	2.02
Vinblastine 0.2μM R03 exp108	2.02
HMS-I1 10μM R06 exp256	2.03
Atovaquone 15μM R08 exp451	2.09
SB743921 0.001μM R05 exp244	2.11
Nocodazole 0.2μM R02 exp64	2.14
Mitomycin-C 0.06μM R08 exp503	2.22
R-DABN 8μM R03 exp111	2.27
GSK461364A 0.1μM R03 exp126	2.36
Nocodazole 0.2μM R00 exp24	2.37
UM131593 0.2μM R03 exp106	2.59
Nocodazole 0.1μM R03 exp94	2.65
Vinblastine 0.015μM R05 exp208	2.74
Hinokiflavone 12μM R08 exp487	2.8
SNS-032 15μM R08 exp443	2.8
BI-2536 0.02μM R03 exp96	3.12
DABN racemic mixture R03 exp93	3.13
Ammonium tetrathiomolybdate 10μM R08 exp448	3.19
BI-6727 0.04μM R03 exp98	3.62
Bleomycin 5μM R08 exp459	3.65
Etoposide 0.3μM R05 exp199	3.66

Gene	Correlation
RNMT	0.671
DPAGT1	0.664
TTI2	0.66
CCDC101	0.655
GNB1L	0.648
PELO	0.643
THG1L	0.638
TTI1	0.621
RPTOR	0.614
ALG1	0.601
TELO2	0.598
PMM2	0.597
MED23	0.597
GMPPB	0.597
BUB3	0.594
RTCB	0.588
MLLT1	0.583
GTF3C2	0.573
WDR24	0.571
GFPT1	0.571
ADSL	0.562
AARS	0.56
VHL	0.556
MED4	0.554
ATXN10	0.55
ALG2	0.543
GTF3C4	0.542
ALG13	0.537
TSEN54	0.535
CBFB	0.533
VMP1	0.533
TRIAP1	0.532
DOHH	0.531
ORAOV1	0.529
NAE1	0.522
RPP21	0.517
PAM16	0.516
MTOR	0.514
DHPS	0.51
DR1	0.509
THOC6	0.509
ZBTB11	0.508
DAD1	0.506
MAD2L1	0.505
HAUS8	0.501
RBM14	0.499
DDOST	0.499
FNTA	0.498
ATP5O	0.497
UHRF1	0.497
UAP1	0.496
MAPK14	0.495
PPP4C	0.495
SRP14	0.494
ATP5B	0.494
SLC35B1	0.492
SRP68	0.492
TMEM41B	0.491
OSGEP	0.489
ALG14	0.488
LSMD1	0.486
UBE3A	0.482
WDR59	0.481
EPRS	0.481
SPCS2	0.48
POLR3H	0.479
TRNT1	0.479
YARS	0.478
EXOC4	0.477
EIF4E	0.477
DDX20	0.473
TADA1	0.473
UBE2G2	0.473
CSNK2B	0.471
DBR1	0.47
GTF3C3	0.469
BAP1	0.469
TLE4	0.469
ATP5J	0.468
RPN1	0.468
SEH1L	0.467
HSPD1	0.465
TXNRD1	0.464
VEZT	0.463
SNAPC5	0.462
RFT1	0.461
WDR1	0.46
FH	0.46
UTP23	0.459
FNTB	0.458
TRMT5	0.458
SRP72	0.457
MAX	0.456
MED25	0.455
TAF5L	0.455
ENY2	0.455
OIP5	0.454
GPN2	0.452
EXOC3	0.452
UXT	0.451
PPP2R3C	0.45
WDR82	0.45
MED18	0.449
FLI1	0.448
GEMIN8	0.447
DOLK	0.447
ALG5	0.446
PDAP1	0.445
LAMTOR5	0.445
SPCS3	0.445
SDHA	0.444
CDIPT	0.444
DCTN6	0.444
ZNF259	0.444
ZRSR2	0.443
TGS1	0.442
MAD1L1	0.441
TRMT10A	0.44
SLC25A19	0.44
NARS	0.439
DNAJC3	0.439
LSM11	0.438
DHX29	0.436
DICER1	0.435
PAX5	0.434
SSBP2	0.434
UBA3	0.431
TAF6L	0.43
GTF3C6	0.428
BTAF1	0.427
TMED10	0.426
MAML1	0.426
CCT7	0.426
WDR92	0.425
POLR3B	0.425
TBCB	0.424
XPO6	0.424
TPK1	0.423
TAMM41	0.423
ERCC2	0.421
TIMM22	0.421
DERL2	0.421
GTF2A2	0.42
OTUD5	0.42
DPF2	0.418
GEMIN5	0.418
MEAF6	0.418
PGGT1B	0.416
WARS	0.416
TCF4	0.416
LMO2	0.416
GGNBP2	0.415
ALG6	0.414
THOC3	0.414
ATP5D	0.414
ELAC2	0.414
PHB	0.414
LSM10	0.413
IRF2BP2	0.413
MED13	0.412
ACTR10	0.41
ZNF592	0.41
TPRKB	0.41
TTC4	0.409
DHDDS	0.408
CRCP	0.407
AASDHPPT	0.407
ATP2A2	0.407
TEN1	0.407
SMARCD1	0.407
BRD9	0.406
ATF7IP	0.405
TRIM28	0.405
SCAF8	0.405
MBTPS2	0.405
MED31	0.404
CDC37	0.402
ICMT	0.402

Global Fraction of Cell Lines Where Essential: 2/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	1/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	1/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 30
Expression level (log2 read counts): 5.4

Expression Distribution

WD40
Med16

thyroid hormone receptor coactivator activity
vitamin D receptor binding
thyroid hormone receptor binding
mediator complex
catalytic activity
transcription coregulator activity
transcription initiation from RNA polymerase II promoter
DNA-templated transcription, initiation
transcription coactivator activity
transcription by RNA polymerase II
transcription, DNA-templated
nucleic acid-templated transcription
RNA biosynthetic process
nucleobase-containing compound biosynthetic process
heterocycle biosynthetic process
aromatic compound biosynthetic process
organic cyclic compound biosynthetic process
positive regulation of transcription, DNA-templated
cellular nitrogen compound biosynthetic process
positive regulation of nucleic acid-templated transcription
positive regulation of RNA biosynthetic process
RNA metabolic process
cellular macromolecule biosynthetic process
positive regulation of RNA metabolic process
macromolecule biosynthetic process
positive regulation of nucleobase-containing compound metabolic process
positive regulation of macromolecule biosynthetic process
positive regulation of cellular biosynthetic process
positive regulation of gene expression
membrane
gene expression
positive regulation of biosynthetic process